Biochemistry

1906 Submissions

[3] viXra:1906.0482 [pdf] submitted on 2019-06-25 16:22:14

Purification of Total Flavonoids from Aurea Helianthus Flowers and in Vitro Hypolipidemic Effect

Authors: Hyon-il Ri1, Un-hak Pak1, Myong-su Kang2, Tae-mun Kim2
Comments: 10 Pages.

The effects of purification methods and its hypolipidemic function on the total flavonoids of Aurea Helianthus flower were investigated. Liquid-liquid extraction of ethanol extract from Aurea Helianthus flower was carried out by using different polar solvents. The extract with the highest total flavonoid content was selected, and the optimal conditions for purification of total flavonoids were determined by purification with macroporous resin. The human digestive environment was simulated in vitro, and the binding ability of different flavonoid samples to three kinds of cholate was compared. The results showed that the purity of total flavonoids in ethanol extract was 27.8%, the purity of total flavonoids in ethyl acetate extract was 46.4%, and the purity was increased by 18.6%. Subsequent purification with AB-8 macroporous resin; loading of total flavonoids at a concentration of 5.5 mg/mL, flow rate of 1.5 mL/min, 110 mL; use of 75% ethanol, 80 mL as eluent at a flow rate of 1.5 mL The elution at /min resulted in a total flavonoid purity of 83.5 % and an increase of 37.1%, and a good purification effect was obtained. The binding rate of total flavonoids purified by AB-8 macroporous resin to sodium taurocholate, sodium glycocholate and sodium cholate was 88.2%, 73.2% and 75.8 %, respectively. The binding ability was the strongest, and the others were ethyl acetate. Extract, ethanol extract. The purity of total flavonoids showed a good correlation with the binding capacity of cholate, and the correlation coefficient was between 0.963 and 0.988. The total flavonoids of Aurea Helianthus flower have good bile acid binding ability and can be used as the focus of natural hypolipidemic substances.
Category: Biochemistry

[2] viXra:1906.0449 [pdf] submitted on 2019-06-23 11:51:30

A Glimpse on Tuberculosis and Scope of Inhalable Anti Tubercular Therapy

Authors: Vishnu Vardhan Reddy Beeram, Venkata Nadh Ratnakaram
Comments: 20 Pages.

A glimpse on tuberculosis and scope of inhalable anti tubercular therapy
Category: Biochemistry

[1] viXra:1906.0406 [pdf] submitted on 2019-06-20 18:25:03

Effect of Different Polarity Solvents on Total Phenols and Flavonoids Content, and in-Vitro Antioxidant Properties of Flowers Extract from Aurea Helianthus

Authors: Hyon-il Ri1, Un-hak Pak1, Jong-hyok Yang1, Kwen-il Son1, Yu-il Kim1, Kyong-chol Son1, Chol-jin Ri1, Myong-su Kang2, Tae-mun Kim2
Comments: 7 Pages.

The total phenols and flavonoids content of different polar solvent extracts from Aurea Helianthus flowers, and their antioxidant activity were determined. The ethanol extract of Aurea Helianthus flowers were suspended in water and fractionated using different polar solvents; hexane, chloroform, ethyl acetate, butanol and water. The parameters of each extract mentioned above were determined using Folin-Ciocalteu reagent (FCR) method, AlCl3 colorimetry method, Ferric reducing ability of plasma (FRAP) assay, total antioxidant activity (TAA) assay and DPPH radical scavenging assay. The highest total phenols content (516.21 mg GAE/g) and flavonoids content (326.06 mg QCE /g)were obtained in ethyl acetate extract, the correlation between TPC and TFC assay was found to be 0.967. All polar solvent extracts of Aurea Helianthus flowers showed significant antioxidant effects, the highest inhibition was obtained in ethyl acetate and chloroform extracts and the lowest inhibition in the water extract. There is a good correlation of total phenols and flavonoids content with antioxidant activity. This work indicated that the polar solvent extracts of Aurea Helianthus flowers contain high phenols and flavonoids content, and exhibited antioxidant activities in vitro, therefore, could be candidates for use as natural antioxidant.
Category: Biochemistry