Biochemistry

1701 Submissions

[5] viXra:1701.0691 [pdf] submitted on 2017-01-31 18:17:02

The Time Principle of Life Formation

Authors: Jeffrey Joseph Wolynski
Comments: 1 Page.

For a star to form life in the general theory of stellar metamorphosis, a star needs to evolve over very long periods of time. If the star evolves too fast, life will not form. Explanation and a few examples are provided to explain this principle.
Category: Biochemistry

[4] viXra:1701.0342 [pdf] submitted on 2017-01-10 00:56:43

Modulation of Pro-inflammatory Cytokines Expression of the Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation in Mouse Splenocytes

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, William Dean Plikerd, Peter L. Surguy, Robert John Kock, Rolando Baptista Piedad, Russell Phillip Callas, Sakina A. Ansari, Sandra Lee Barrett, Sara Friedman, Steven Lee Christie
Comments: 10 Pages.

With the increasing popularity of herbomineral preparations in healthcare, a new proprietary herbomineral formulation was formulated with ashwagandha root extract and three minerals viz. zinc, magnesium, and selenium. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation using murine splenocyte cells. The test formulation was divided into two parts. One was the control without the Biofield Energy Treatment. The other part was labelled the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers. Through MTT assay, all the test formulation concentrations from 0.00001053 to 10.53 µg/mL were found to be safe with cell viability ranging from 102.61% to 194.57% using splenocyte cells. The Biofield Treated test formulation showed a significant (p≤0.01) inhibition of TNF-α expression by 15.87%, 20.64%, 18.65%, and 20.34% at 0.00001053, 0.0001053, 0.01053, and 0.1053, µg/mL, respectively as compared to the vehicle control (VC) group. The level of TNF-α was reduced by 8.73%, 19.54%, and 14.19% at 0.001053, 0.01053, and 0.1053 µg/mL, respectively in the Biofield Treated test formulation compared to the untreated test formulation. The expression of IL-1β reduced by 22.08%, 23.69%, 23.00%, 16.33%, 25.76%, 16.10%, and 23.69% at 0.00001053, 0.0001053, 0.001053, 0.01053, 0.1053, 1.053 and 10.53 µg/mL, respectively compared to the VC. Additionally, the expression of MIP-1α significantly (p≤0.001) reduced by 13.35%, 22.96%, 25.11%, 22.71%, and 21.83% at 0.00001053, 0.0001053, 0.01053, 1.053, and 10.53 µg/mL, respectively in the Biofield Treated test formulation compared to the VC. The Biofield Treated test formulation significantly down-regulated the MIP-1α expression by 10.75%, 9.53%, 9.57%, and 10.87% at 0.00001053, 0.01053, 0.1053 and 1.053 µg/mL, respectively compared to the untreated test formulation. The results showed the IFN-γ expression was also significantly (p≤0.001) reduced by 39.16%, 40.34%, 27.57%, 26.06%, 42.53%, and 48.91% at 0.0001053, 0.001053, 0.01053, 0.1053, 1.053, and 10.53 µg/mL, respectively in the Biofield Treated test formulation compared to the VC. The Biofield Treated test formulation showed better suppression of IFN-γ expression by 15.46%, 13.78%, 17.14%, and 13.11% at concentrations 0.001053, 0.01053, 0.1053, and 10.53 µg/mL, respectively compared to the untreated test formulation. Overall, the results demonstrated that The Trivedi Effect®- Biofield Energy Healing (TEBEH) has the capacity to potentiate the immunomodulatory and anti-inflammatory activity of the test formulation. Biofield Energy may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.
Category: Biochemistry

[3] viXra:1701.0015 [pdf] submitted on 2017-01-03 01:26:13

Effect of Biofield Energy Healing Based Herbomineral Formulation on Pro-inflammatory Cytokines Expression in Biofield Treated Mouse Splenocyte Cells: Impact of the Trivedi Effect®

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Barry Dean Wellborn, Deborah Lea Smith, Dezi Ann Koster, Elizabeth Patric, Jagdish Singh, Kathleen Starr Vagt, Krista Joanne Callas, Olga Mirgalijeva
Comments: 10 Pages.

A proprietary herbomineral formulation was formulated with four ingredients; a mixture of the minerals (zinc, magnesium, and selenium) and the herbal root extract ashwagandha. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation in splenocyte cells, which were isolated from Biofield Treated mice. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from seven renowned Biofield Energy Healers. The splenocyte cells were treated with the test formulation at concentrations ranges from 0.00001053 to 10.53 µg/mL and analyzed after 48 hours of treatment by MTT assay. The cell viability data showed safe concentrations up to 1.053 µg/mL with viability ranges from 69.22% to 123.88% in the test formulation groups. The expression of TNF-α was decreased by 4.82% at 1.053 µg/mL in the Biofield Energy Treated test formulation compared with the vehicle control. The level of TNF-α was significantly decreased by 2.02%, 4.92%, and 18.78% at 0.00001053, 0.001053, and 1.053 µg/mL, respectively in the Biofield Energy Treated test formulation group as compared to the untreated test formulation. The expression of IL-1β was significantly reduced by 83.65%, 92.15%, 27.30%, and 41.88% at 0.00001053, 0.0001053, 0.001053, and 1.053 µg/mL, respectively in the Biofield Energy Treated test formulation compared with the vehicle control. The Biofield Treated test formulation showed significant reduction of IL-1β by 17.26%, 92.61% (p≤0.001), 34.62% (p≤0.05), and 16.13% at 0.00001053, 0.0001053, 0.001053, and 1.053 µg/mL, respectively compared with the untreated test formulation. Additionally, the expression of chemokine MIP-1α was significantly reduced by 17.03%, 10.99%, 22.33%, 24.21%, 21.61%, and 30.67% at 0.00001053, 0.0001053, 0.001053, 0.01053, 0.1053, and 1.053 µg/mL, respectively in the Biofield Treated test formulation compared with the vehicle control. The MIP-1α expression was significantly reduced by 19.32% and 12.56% at 0.01053 and 0.1053 µg/mL, respectively in the Biofield Treated test formulation compared with the untreated test formulation. The overall results demonstrated that the Biofield Energy Treated test formulation significantly down-regulated the expression of TNF-α, IL-1β, and MIP-1α in the Biofield Treated mice splenocyte cells compared to the untreated test formulation. These data suggest that the Biofield Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.
Category: Biochemistry

[2] viXra:1701.0005 [pdf] submitted on 2017-01-02 04:09:13

Evaluation of Pro-Inflammatory Cytokines Expression in Mouse Splenocyte Cells After Incubation with the Biofield Energy Healing Based Herbomineral Formulation: Influence of the Trivedi Effect®

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Barry Dean Wellborn, Deborah Lea Smith, Dezi Ann Koster, Elizabeth Patric, Jagdish Singh, Kathleen Starr Vagt, Krista Joanne Callas, Olga Mirgalijeva
Comments: 10 Pages.

The use of herbomineral formulations in the healthcare sector has increased due to their high safety and better therapeutic action. A new proprietary herbomineral formulation was formulated with a combination of the herbal root extract ashwagandha and minerals viz. zinc, magnesium, and selenium. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the test formulation in splenocytes. The test formulation was divided into two parts; one was the control without the Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers. The MTT assay showed that the test formulation exhibited safe concentrations up to 1.053 µg/mL with cell viability ranging from 88.19% to 110.30% in the Biofield Energy Treated sample. The Biofield Energy Healing significantly enhanced the cell viability as compared with the untreated test formulation. The expression of TNF-α was significantly inhibited by 7.23% at 1.053 µg/mL in the Biofield Energy Treated test formulation compared with the vehicle control. The level of TNF-α was significantly decreased by 3.90%, 11.74%, 3.12%, and 9.17% at 0.001053, 0.01053, 0.1053 and 1.053 µg/mL, respectively in the Biofield Energy Treated test formulation compared with the untreated test formulation. Additionally, the expression of IL-1β was significantly reduced by 28.98%, 51.23%, 53.06%, 48.98%, 55.71%, and 59.10% at 0.00001053, 0.0001053, 0.001053, 0.01053, 0.1053, and 1.053 µg/mL, respectively in the Biofield Energy Treated test formulation compared with the vehicle control. Further, the Biofield Treated test formulation showed significant reduction of IL-1β by 35.07% (p≤0.05), 47.46% (p≤0.05), and 57.51% (p≤0.01) at 0.001053, 0.1053, and 1.053 µg/mL, respectively compared with the untreated test formulation. Similarly, the MIP-1α expression was inhibited by the Biofield Energy Treated formulation and showed immunosuppressive activity at 0.00001053, 0.0001053, 0.001053, and 0.01053 µg/mL by 19.38%, 24.97%, 31.23%, and 25.41%, respectively compared with the vehicle control group. The Biofield Treated test formulation significantly (p<0.001) reduced the MIP-1α expression by 19.33%, 22.57%, and 30.50% at 0.0001053, 0.001053, and 0.01053 µg/mL, respectively compared with the untreated test formulation. Overall, The Trivedi Effect®- Biofield Energy Healing (TEBEH) significantly down-regulated the pro-inflammatory cytokines and potentiated the immunosuppressive effect of the Biofield Energy Treated test formulation, which can be better utilized for organ transplants, autoimmune diseases, inflammatory disorders, anti-aging, stress management, overall health and quality of life, etc.
Category: Biochemistry

[1] viXra:1701.0004 [pdf] submitted on 2017-01-02 04:10:25

Evaluation of Pro-Inflammatory Cytokines Expression in Mouse Splenocytes After Co-Incubation with the Biofield Energy Treated Formulation: Impact of the Trivedi Effect®

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Michael Peter Ellis, James Jeffery Peoples, James Joseph Meuer, Johanne Dodon, John Lawrence Griffin, John Suzuki, Joseph Michael Foty, Judy Weber, Julia Grace McCammon
Comments: 10 Pages.

With the increasing popularity of herbomineral preparations in healthcare, a new proprietary herbomineral formulation was formulated with ashwagandha root extract and minerals viz. zinc, magnesium, and selenium. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral test formulation using mice splenocytes. The test formulation was divided into two parts. One part was the control without the Biofield Treatment. The other part was labelled the Biofield Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. The splenocyte cells were exposed with the test formulation at ranges of 0.00001053 to 10.53 µg/mL for cell viability by MTT assay, with cell viability ranging from 77.50% to 176.52%. TNF-α was significantly inhibited by 15.88%, 15.28%, 12.30%, 12.60%, and 22.72% at 0.00001053, 0.001053, 0.1053, 1.053, and 10.53 µg/mL, respectively in the Biofield Treated test formulation compared to the vehicle control (VC). TNF-α was significantly reduced by 2.33% and 8.35% at 1.053 and 10.53 µg/mL, respectively compared to the untreated test formulation. IL-1β was significantly reduced by 30.81%, 27.36%, 23.92%, 18.40%, 11.27%, and 21.16% at 0.00001053, 0.0001053, 0.001053, 0.01053, 0.1053, and 1.053 µg/mL, respectively in the Biofield Treated test formulation compared to the VC. IL-1β was significantly reduced by 48.63% (p≤0.001) and 15.28% at 0.00001053 and 0.0001053 µg/mL, respectively in the Biofield Treated test formulation compared to the untreated test formulation. MIP-1α expression was inhibited by the Biofield Treated test formulation and showed immunosuppressive activity at 0.01053, 0.1053, 1.053, and 10.53 µg/mL by 22.33%, 16.25%, 15.58%, and 21.83%, respectively compared to the VC. The Biofield Treated test formulation significantly reduced the MIP-1α expression by 13.27% and 15.67% (p<0.05) at 0.01053 and 10.53 µg/mL, respectively compared to the untreated test formulation. The results showed the expression of IFN-γ was significantly reduced by 33.45%, 25.38%, 37.15%, 27.74%, 32.44%, 23.03%, and 44.21% at 0.00001053, 0.0001053, 0.001053, 0.01053, 0.1053, 1.053, and 10.53 µg/mL, respectively in the Biofield Treated test formulation compared to the VC. Further, the IFN-γ level was significantly decreased by 19.02% at 10.53 µg/mL in the Biofield Treated test formulation compared to the untreated test formulation. Overall, the results demonstrate that The Trivedi Effect® Biofield Energy Healing (TEBEH) significantly enhanced the anti-inflammatory and immunomodulatory properties of the treated formulation, and may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.
Category: Biochemistry