A Validated RP-HPLC Method for the Estimation of Melphalan in Tablet Dosage Forms

Authors: K.Kiran Kumar1, R. Venkata Nadh

A simple, selective, rapid, precise and economical reverse phase HPLC method has been developed for the determination of melphalan in tablet. The analyte was resolved by using a mobile phase (Acetonitrile, water and 1% ortho phosphoric acid in the ratio 70:27:3 v/v/v) at a flow rate 1 ml/min on an isocratic HPLC system (PEAK) consisting UV-Visible detector, ODS C-18, RP column (4.6 mm i.d x250 mm) at a wavelength of 275 nm. The linear dynamic range for melphalan was 2.0 μg/mL – 14.0μg/mL. The limit of detection [LOD] and Limit of quantification [LOQ] for melphalan was 0.5μg/mL and 1.5μg/mL respectively.

Comments: 5 Pages. OK

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[v1] 2019-05-19 06:02:31

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