Biochemistry

   

Study the Regulation of the “nos” Genes of Bradyrhizobium Japonicum

Authors: Alicia Abarca Cifuentes, Maria Jesus Delgado

The goal of this Project is to study the regulation of the “nos” genes of Bradyrhizobium japonicum, which encodes the nitrous oxide reductase enzyme responsible for the reduction of the greenhouse gas nitrous oxide (N2O). To achieve this goal, I am using transcriptional fusions of the promoter regions of “nos” genes to lacZ reporter gene I have transferred by conjugation a nosRlacZ fusion into the parental strain USDA110 and a-mutant strain lacking the regulatory protein RegR. The transconjugants obtained were grown in minima media with different concentrations of oxygen. Cells have been obtained by centrifugation and I have measured the beta-Galactosidase activity of cells.

Comments: Alicia Abarca Cifuentes as Final project student, 26 Pages.

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Submission history

[v1] 2015-10-19 10:38:49

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