Chemistry

1707 Submissions

[26] viXra:1707.0256 [pdf] submitted on 2017-07-19 00:46:58

A Comprehensive Physicochemical, Thermal, and Spectroscopic Characterization of Zinc (Ii) Chloride Using X-Ray Diffraction, Psd, Dsc, Thermogravimetric Analysis/dta, Ultraviolet-Visible, and Fourier Transform-Infrared Spectroscopy

Authors: Mahendra Kumar Trivedi
Comments: 8 Pages.

Objective: Zinc chloride is an important inorganic compound used as a source of zinc and has other numerous industrial applications. Unfortunately, it lacks reliable and accurate physicochemical, thermal, and spectral characterization information altogether. Hence, the authors tried to explore in-depth characterization of zinc chloride using the modern analytical technique. Materials and Methods: The analysis of zinc chloride was performed using powder X-ray diffraction (PXRD), particle size distribution, differential scanning calorimetry (DSC), thermogravimetric analysis/differential thermogravimetric analysis (TGA/DTG), ultraviolet-visible spectroscopy (UV-vis), and Fourier transform-infrared (FT-IR) analytical techniques. Results: The PXRD patterns showed well-defined, narrow, sharp, and the significant peaks. The crystallite size was found in the range of 14.70–55.40 nm and showed average crystallite size of 41.34 nm. The average particle size was found to be of 1.123 (d10), 3.025 (d50), and 6.712 (d90) μm and average surface area of 2.71 m2/g. The span and relative span values were 5.849 μm and 1.93, respectively. The DSC thermogram showed a small endothermic inflation at 308.10°C with the latent heat (ΔH) of fusion 28.52 J/g. An exothermic reaction was observed at 449.32°C with the ΔH of decomposition 66.10 J/g. The TGA revealed two steps of the thermal degradation and lost 8.207 and 89.72% of weight in the first and second step of degradation, respectively. Similarly, the DTG analysis disclosed Tmaxat 508.21°C. The UV-vis spectrum showed absorbance maxima at 197.60 nm (λmax), and FT-IR spectrum showed a peak at 511/cm might be due to the Zn–Cl stretching. Conclusions: These in-depth, comprehensive data would be very much useful in all stages of nutraceuticals/pharmaceuticals formulation research and development and other industrial applications.
Category: Chemistry

[25] viXra:1707.0183 [pdf] submitted on 2017-07-13 07:24:00

Physicochemical, Thermal and Spectroscopic Characterization of Sodium Selenate Using XRD, PSD, DSC, TGA/DTG, UV-vis, and FT-IR

Authors: Mahendra Kumar Trivedi
Comments: 8 Pages.

Sodium selenate is an important inorganic compound but lacks reliable and accurate physico-chemical and spectral characterization information. The study was carried out for the in-depth physicochemical, thermal, and spectroscopic characterization of sodium selenate using various analytical techniques. The powder X-ray diffraction spectroscopy analysis showed well-defined and sharp peaks indicated sodium selenate is crystalline in nature. The crystallite size was found to be in the range of 28.75 to 49.97 nm. The average particle size was found to be of 3.93 (d10), 14.44 (d50), and 40.648 (d90) µm with an average surface area of 0.676 m2/g. The differential scanning calorimetry showed the endothermic inflation at 588.81 °C with the latent heat of fusion 103 J/g. The thermogravimetric analysis revealed two steps of the thermal degradation process. Similarly, the differential thermogravimetric analysis exhibited the major peaks in the thermogram and disclosed Tmax at 852.65 °C. The UV-visible spectrum showed maximum absorbance at 205.1 nm (λmax). The Fourier transform infrared spectrum showed a peak at 888 cm-1 due to the Se-O stretching. This information would be very much useful in the field of nutraceuticals/ pharmaceuticals and other industries using sodium selenate as an ingredient.
Category: Chemistry

[24] viXra:1707.0182 [pdf] submitted on 2017-07-13 07:25:03

Effect of a Novel Ashwagandha-Based Herbomineral Formulation on Pro-Inflammatory Cytokines Expression in Mouse Splenocyte Cells: a Potential Immunomodulator

Authors: Mahendra Kumar Trivedi
Comments: 5 Pages.

Background: Herbomineral formulations are momentous in an audience of worldwide by virtue of their holistic approach to life. These formulations are widely used as complementary therapies in immunocompromised patients including cancer. Still, there is the need of cost-effective and safe herbomineral-based formulation that can modulate immune response by the regulation of cytokines cascades. Objective: Current study, we investigated immunomodulatory effect of TEBEH in LPS-induced cytokines expression levels in mouse splenocytes in vitro. Materials and Methods: The most effective and safe concentrations of TEBEH were chosen by determining the cell viability of splenocytes using MTT assay. The pro-inflammatory cytokines such as TNF-α, IL-1β, MIP-1α, and IFN-γ were measured in cell supernatants using ELISA. Results: MTT data showed TEBEH formulation was found safe up to 10.53 µg/mL. At noncytotoxic concentrations (0.00001053–10.53 µg/mL), TEBEH significantly (P ≤ 0.001) inhibited the expressions of TNF-α, IL-1β, and MIP-1α in mouse splenocytes as compared with vehicle control. Conclusion: In summary, TEBEH may indeed promote an anti-inflammatory environment by suppression of pro-inflammatory cytokines. These observations indicated that TEBEH has potential effects in downregulating the immune system and might be developed as a useful anti-inflammatory product for various inflammatory disorders. Summary: The present study was undertaken to evaluate an immunomodulatory effect of the herbomineral formulation in LPS-induced mouse splenocytes with the measurement of cytokines expression such as TNF-α, IL-1β, MIP-1α and IFN-γ. The results showed that the expression of TNF-α, IL-1β, and MIP-1α was significantly down-regulated while, IFN-γ was significantly up-regulated in mouse splenocytes. It is hypothesized that modulation of the proinflammatory cytokines might occur via NF-kB pathway. Therefore, the herbomineral test formulation might act as an effective anti-inflammatory and immunomodulatory product, and this can be used as a complementary and alternative treatment for the prevention of various types of inflammatory and auto-immune disorders.
Category: Chemistry

[23] viXra:1707.0101 [pdf] submitted on 2017-07-07 01:43:14

Overall Skin Health Potential of the Biofield Energy Healing Based Herbomineral Formulation Using Various Skin Parameters

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Janice Patricia Kinney
Comments: 10 Pages.

The aim of the present study was to evaluate the effect of the Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation and medium (DMEM) against various skin health parameters using three cell lines i.e., HFF-1, HaCaT, and B16-F10. The various study parameters viz. collagen, elastin, hyaluronic acid, melanin, cell viability against UV-B induced stress, and wound healing were evaluated. The test formulation and DMEM were divided into two parts. One part of the test formulation and one part of the DMEM received the Consciousness Energy Healing Treatment by Janice Patricia Kinney and were defined as the Biofield Energy Treated samples, while the other parts were denoted as the untreated test samples. Cell viability using MTT assay showed more than 70% cells were viable in all the tested concentrations in three cells, indicating that the test formulation was safe and nontoxic. The collagen synthesis was significantly (p≤0.001) increased by 22.42% and 17.48% in the UT-DMEM + BT-Test formulation group at 0.63 and 2.5 µg/mL, respectively in relation to the UT-DMEM + UT-Test formulation group. The Elastin level was significantly (p≤0.001) increased by 28.41% in the BT-DMEM + BT-Test formulation group at 5 µg/mL compared to the untreated group. Hyaluronic acid at 0.63 µg/mL was increased significantly by 15.90% in the BT-DMEM + BT-Test formulation group compared to the untreated group. The level of melanin was reduced significantly by 9.25% and 7.26% in the BT-DMEM + BT-Test formulation group at 0.013 and 0.063 µg/mL, respectively in relation to the untreated group. Protection of skin cells after UV-B exposure data displayed that the cell viability was increased significantly by 17.88%, 20.10%, and 25.77% in the BT-DMEM + BT-Test formulation group at 0.625, 1.25, and 2.5 µg/mL, respectively compared to the UT-DMEM + UT-Test formulation group. Wound healing data exhibited significant wound closure and cell migration activities in the HFF-1 and HaCaT cells compared to the UT-DMEM + UT-Test formulation. Overall, the data suggests that the Biofield Energy Treated DMEM and test formulation demonstrated better responses compared to the untreated medium and test formulation with respect to the tested skin health parameters. Therefore, the Biofield Energy Healing and the Treated test formulation could be developed as an effective cosmetic product to protect and treat the various skin problems including infection, photosensitivity, erythema, contact dermatitis, seborrheic dermatitis, athlete's foot, psoriasis, erythema, cutis rhomboidalis nuchae, skin aging, wrinkles and/or change in skin color, etc.
Category: Chemistry

[22] viXra:1707.0100 [pdf] submitted on 2017-07-07 01:43:59

The Study of Biofield Energy Treatment Based Herbomineral Formulation in Skin Health and Function

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Johanne Dodon
Comments: 12 Pages.

The aim of the study was to evaluate the effect of the The Trivedi Effect®- Consciousness Energy Healing Treatment based new proprietary herbomineral formulation and cell medium (DMEM) on various skin health parameters using HFF-1, HaCaT, and B16-F10 cell lines. It contained minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, along with the mixture of Centella asiatica extract and tetrahydrocurcumin (THC). The test formulation and DMEM were divided into two parts each, one part of each was coded as untreated (UT), while the second part of each was treated with the Biofield Treatment by Johanne Dodon and denoted as the Biofield Energy Treated (BT) test items. MTT assay result showed that the test formulation was safe and nontoxic up to 80 µg/mL concentration. The fibroblast cell proliferation assay using BrdU method showed a significant increased cell proliferation at 2.13, 8.75, and 17.53 µg/mL by 3.90%, 14.68% and 29.54%, respectively in the BT-DMEM + BT-Test formulation group compared to the untreated group. The collagen data showed an increased synthesis by 7.42% and 3.84% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL with respect to the untreated group. Similarly, the elastin synthesis was increased by 19.8% and 25.8% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 5 µg/mL with respect to the untreated group. However, the Biofield Energy Treated test formulation showed an increased level of hyaluronic acid by 1.7% in UT-DMEM + BT-Test formulation group compared to the untreated group. Melanin synthesis inhibition was observed by 15.21% and 11.43% in UT-DMEM + BT-Test Formulation and BT-DMEM + BT-Test Formulation groups, respectively at 0.125 µg/mL compared with the untreated group in B16-F10 melanoma cell line. Anti-wrinkling effects showed an improved cell viability by 7.65% at 1.25 µg/mL in the BT-DMEM + BT-Test formulation experimental group compared with the untreated test group. In addition, the Biofield Energy Treated Test formulation/DMEM showed a significant wound healing activity and high cellular migration of fibroblast and keratinocytes in HFF-1 and HaCaT cells, respectively. Overall, the data suggested that Biofield Treated formulation/DMEM could be helpful for skin healing, improvement the skin health, elasticity, and tightness, as well as, improving skin health issues such as wrinkling, pigmentation disorders, redness, swelling, skin cancer, aging, rashes, and skin sagging.
Category: Chemistry

[21] viXra:1707.0099 [pdf] submitted on 2017-07-07 01:44:56

Skin Nourishing Effects of Biofield Energy Healing Treatment Based Herbomineral Formulation

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Shirley Theresa Holmlund
Comments: 10 Pages.

The aim of the present study was undertaken to evaluate the effects of a Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation on skin health. The test formulation and DMEM were separated into two parts. One part of each received the Consciousness Energy Healing Treatment by Shirley Theresa Holmlund and were termed as the Biofield Energy Treated samples, while the other parts were considered as the untreated test samples. MTT assay showed more than 70% cells were viable in all the tested concentrations in three cell lines and that the test formulation was safe and nontoxic. The percent cell proliferation using BrdU assay was significantly increased by 343.57%, 100.29%, and 132.6% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 8.75 µg/mL compared to the UT-DMEM + UT-Test formulation group. The collagen synthesis was significantly increased by 5.94% and 10.84% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL with respect to the untreated group. Elastin level was significantly (p≤0.001) increased by 15.95%, 58.31%, and 47.18% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively compared to the untreated group. The level of hyaluronic acid was significantly increased by 15.13% and 68.56% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL compared to the untreated group. The level of melanin was significantly reduced by 17.06%, 6.94%, and 13.07% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.063 µg/mL compared to untreated group. The effect of skin protection upon exposure with UV-B rays displayed some alteration of the percent cell viability in all the tested groups. The wound healing activity by scratch assay exhibited significant wound closure and cell migration in the test formulation and DMEM in HFF-1 cells compared to the untreated group. Altogether, the data suggests that the Consciousness Energy Healing Treated DMEM and test formulation exhibited better skin protective responses compared to the untreated DMEM and test formulation group with respect to the skin health parameters. Therefore, the Biofield Energy Treated test formulation could be developed as a potential cosmetic product against various skin problems viz. eczema, vitiligo, psoriasis, acne, hives, rosacea, ichthyosis, seborrheic dermatitis, erythema, contact dermatitis, skin aging, wrinkles, etc.
Category: Chemistry

[20] viXra:1707.0097 [pdf] submitted on 2017-07-07 01:47:41

The Use of Consciousness Energy Healing Based Herbomineral Formulation for Skin Anti-Aging Strategies

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Vaibhav Rajan Parulkar
Comments: 11 Pages.

The present study was undertaken to evaluate the effect of a Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation and medium (DMEM) against skin health parameters using HFF-1, HaCaT, and B16-F10 cells. The test formulation and DMEM were divided into two parts. One part of the test formulation and one part of the DMEM received the Consciousness Energy Healing Treatment by Vaibhav Rajan Parulkar and were defined as the Biofield Energy Treated samples, while the other parts were denoted as the untreated test samples. Cell viability using MTT assay showed more than 75% cells were viable in all the tested concentrations in three cells, indicating that the test formulation was safe and nontoxic. The percent cell proliferation by BrdU assay was significantly increased by 434.14%, 244.77%, and 268.53% in the UT-DMEM + BT-Test Formulation, BT-DMEM + UT-Test Formulation, and BT-DMEM + BT-Test Formulation groups, respectively at 8.75 µg/mL in relation to the UT-DMEM + UT-Test Formulation group. Elastin was significantly (p≤0.001) increased 93.52% and 75.81% in the UT-DMEM + BT-Test Formulation and BT-DMEM + UT-Test Formulation groups, respectively at 10 µg/mL compared to the UT-DMEM + UT-Test Formulation group. Hyaluronic acid was significantly increased by 100.07% (p≤0.05), 41.60%, and 50.10% in the UT-DMEM + BT-Test Formulation, BT-DMEM + UT-Test Formulation, and BT-DMEM + BT-Test Formulation groups, respectively at 0.625 µg/mL in relation to the UT-DMEM + UT-Test Formulation group. Melanin was decreased by 10.09% and 6.51% in the UT-DMEM + BT-Test Formulation and BT-DMEM + UT-Test Formulation groups, respectively at 0.125 µg/mL compared to the UT-DMEM + UT-Test Formulation group. Protection of skin cells after UV-B exposure data displayed that the cell viability was increased by 7.22%, 5.75%, and 8.15% in the BT-DMEM + BT-Test Formulation at 0.625%, 1.25%, and 2.5% µg/mL, respectively compared to the UT-DMEM + UT-Test Formulation group. Wound healing data exhibited significant wound closure and cell migration activities in the HFF-1 cells compared to the UT-DMEM + UT-Test formulation group. Overall, the data suggests that the Biofield Energy Treated DMEM and test formulation demonstrated better skin protection action compared to the untreated DMEM and test formulation. Therefore, the Biofield Energy Treated test herbomineral formulation could be useful for the development of effective cosmetic products for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles and/or change in the skin color, etc.
Category: Chemistry

[19] viXra:1707.0096 [pdf] submitted on 2017-07-07 01:48:10

An in Vitro Study of Biofield Energy Healing Based Herbomineral Formulation for Skin Protection

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Eileen Mary Meagher
Comments: 11 Pages.

The current research work deals with the impact of the Consciousness Energy Healing Treatment (The Trivedi Effect®) based herbomineral test formulation and cell medium (DMEM) against various skin health parameters. The test formulation is the combination of minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two equal parts, i.e. one part received the Biofield Energy Treatment by Eileen Mary Meagher and was labeled as the Biofield Treated (BT) samples, while other represented was defined as the untreated test (UT) samples. The MTT assay showed that the test formulation was found safe and nontoxic with greater than 75% cell viability. Cell proliferation assay using BrdU method showed an improved proliferation rate by 343.23%, 316.13%, and 512.75% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 17.5 µg/mL, compared with the untreated group. The collagen level was significantly increased by 15.70% and 13.52% at 2.5 and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The level of elastin was increased by 47.0% and 64.1% at 2.5 and 1.25 µg/mL, respectively in the BT-DMEM + UT-Test formulation, group compared with the untreated group. Hyaluronic acid synthesis was increased by 4.37%, 41.47%, and 19.74% at 2.5, 1.25, and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation group with respect to the untreated group. Melanin synthesis was significantly inhibited by 8.83%, 5.78%, and 12.68% at 0.0125, 0.0625, and 0.125 µg/mL, respectively in the UT-DMEM + BT-Test formulation group compared with the untreated group. Anti-wrinkling effect showed an improved cell viability by 4.32% and 2.47% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT- Test formulation groups, respectively in HFF-1 cells compared with the untreated group. Wound healing activity using scratch assay exhibited improved a healing rate up to 6% in the HFF-1 and HaCaT cells lines in the Biofield Energy Treated Test formulation. In conclusion, results suggest that The Trivedi Effect®- Consciousness Energy Healing Treatment based test formulation and medium have the ability to improve the skin health and can be used as skincare products cosmetics for anti-ageing, anti-wrinkling action and for the treatment of many skin diseases such as Eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, Rosacea, psoriasis, seborrheic dermatitis, skin cancer, etc.
Category: Chemistry

[18] viXra:1707.0084 [pdf] submitted on 2017-07-06 02:19:34

Skin Protective Activity of Consciousness Energy Healing Treatment Based Herbomineral Formulation

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Dennille Mellesia Smith
Comments: 10 Pages.

The current study was attempted to evaluate the impact of the Consciousness Energy Healing (The Trivedi Effect®) Treatment based herbomineral test formulation and cell medium (DMEM) against skin health. The test formulation and DMEM were divided into two parts. One of each part was received the Consciousness Energy Healing Treatment by Dennille Mellesia Smith and was termed as the Biofield Energy Treated samples, while the other parts were denoted as the untreated test items. MTT showed >78% viable cells, indicating that the test formulation was safe and nontoxic in all the tested concentrations in three cell lines. The percent cell proliferation by BrdU assay was significantly increased by 238.30%, 192.06%, and 43.96% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 17.5 µg/mL with respect to the UT-DMEM + UT-Test formulation group. The level of collagen was significantly increased by 55.55%, 32.65%, and 52.48% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 1.25 µg/mL compared to the untreated group. Elastin was significantly (p≤0.001) increased by 6.30%, 105.04%, and 29.41% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 10 µg/mL compared to the untreated group. Hyaluronic acid was increased by 4.78%, 29.71%, and 58.29% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.63 µg/mL compared to the UT-DMEM + UT-Test Formulation group. The level of melanin was reduced by 14.64% and 18.25% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation, respectively at 0.13 µg/mL compared to the untreated group. Skin protection against UV-B data displayed that cell proliferation was increased by 17.88%, 20.14%, and 9.89% in the BT-DMEM + BT-Test formulation at 0.63, 1.25, and 2.5 µg/mL, respectively compared to the untreated group. Wound healing activity exhibited significant wound closure and cell migration in all the tested groups compared to the untreated group. Overall, result suggests that the Biofield Energy Treated DMEM and test formulation exhibited better responses compared to the untreated medium and test formulation. Therefore, the Biofield Energy Treated herbomineral formulation could be useful for the development of an effective cosmetic product for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles and/or change in the skin color, etc.
Category: Chemistry

[17] viXra:1707.0083 [pdf] submitted on 2017-07-06 02:20:14

Biofield Energy Healing Based Herbomineral Formulation: An Emerging Frontier in Cosmetic Medicine

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Dezi Ann Koster
Comments: 11 Pages.

The application of the herbomineral formulations in general skin health are increasing day-by-day due to the excellent outcomes without any adverse effects. This study was designed to evaluate the influence of The Trivedi Effect®-Consciousness Energy Healing Treatment on an herbomineral formulation and cell medium against various skin health parameters. The test formulation consists of minerals (zinc chloride, sodium selenate, and sodium molybdate) and L-ascorbic acid along with herbal extracts, Centella asiatica, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two equal parts, one was treated with a Biofield Treatment (BT) by Dezi Ann Koster and denoted as treated, while the other part was coded as the untreated (UT) groups. MTT assay results showed that test formulation was safe and nontoxic with more than 89% cell viability in the tested cell lines. BrdU assay showed an improved cell proliferation by 2.82% in the UT-DMEM + BT-Test formulation group compared with the untreated group. The level of collagen was significantly increased by 32.42%, 33.64%, and 29.13% at 2.5, 1.25 and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation, while34.17%, 26.73%, and 17.56% increased at 2.5, 1.25 and 0.625 µg/mL, respectively in BT-DMEM + BT-Test formulation group compared with the untreated group. Elastin level was increased by 408.6% at concentration of 0.625 µg/mL in UT-DMEM + BT-Test formulation group compared with untreated group. However, hyaluronic acid (HA) level was increased by 31.88%, 15.52%, and 58.29% at 2.5, 1.25, and 0.625 µg/mL, respectively in the BT-DMEM + BT-Test formulation group compared with untreated group. Besides, melanin synthesis was significantly inhibited by 16.09% and 18.93% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 0.125 µg/mL compared with the untreated group. Anti-wrinkling activity in HFF-1 cells showed an improved cell viability by 5.49% and 11.26% at 1.25 and 0.625 µg/mL, respectively in BT-DMEM + BT-Test formulation group compared with the untreated group. Wound healing scratch assay results showed a significant healing rate by 5% and 10% in HFF-1 and HaCaT cells lines, respectively with high cellular migration of fibroblast and keratinocytes. Overall, it can be concluded that the Biofield Energy Healing (The Trivedi Effect®) based test formulation and cell medium could be helpful against various skin disorders and can be used in psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating agent.
Category: Chemistry

[16] viXra:1707.0082 [pdf] submitted on 2017-07-06 02:20:56

Evaluation of Skin Protective Effect Using in Vitro Cell Line Models of Biofield Energy-Consciousness Energy Healing Treated Herbomineral Formula

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, William Dean Plikerd
Comments: 11 Pages.

Alternative or natural systems of medicine are practiced worldwide that mostly emphasize research of herbal formulations, which might be helpful against various disorders. The objective of this study was to evaluate the influence of The Trivedi Effect®-Consciousness Energy Healing Treatment on an herbomineral test formulation and cell medium (DMEM) for the skin health. The formulation contained minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two equal parts. One part received Biofield Energy Treatment (BT) by William Dean Plikerd and other part was denoted as the untreated test items (UT). MTT assay showed that the test formulation was found safe and nontoxic with greater than 75% cell viability against various tested concentrations. The cell proliferation using BrdU method showed improved cell proliferation by 43.63% and 10.97% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation group, respectively at 17.5 µg/mL compared with the untreated group. The collagen level was significantly increased by 2.65% and 23.56% at 2.5 and 1.25 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The elastin level was increased by 8.34% at a concentrations of 2.5 µg/mL in the BT-DMEM + BT-Test formulation group compared with the untreated group. However, the hyaluronic acid (HA) level was increased by 2.60% at 0.625 µg/mL in the BT-DMEM + UT-Test formulation group compared with the untreated group. Moreover, melanin synthesis was decreased by 14.22% and 17.61% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at a concentration of 0.125 µg/mL. Anti-wrinkling activity in HFF-1 cells showed improve cell viability by 14.54% and 11.32% at 1.25 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively compared with the untreated group. Wound healing scratch assay results showed significantly increased healing rates by 10.5% and 8.3% in the HFF-1 and HaCaT cells lines, respectively in the Biofield Energy Healing based test formulation. In conclusion, The Trivedi Effect® based test formulation and DMEM could be useful to improve the skin ECM components, along with protection against UV-B rays, which could be useful for the development of an effective cosmetic product for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles and/or change in the skin color, etc. as an anti-ageing, skin-whitening, and anti-wrinkling.
Category: Chemistry

[15] viXra:1707.0081 [pdf] submitted on 2017-07-06 02:21:33

Consciousness Energy Healing Treatment Based Herbomineral Formulation: A Safe and Effective Approach for Skin Health

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Jagdish Singh
Comments: 10 Pages.

Oxidative stress causes serious skin damage that is characterized by ageing, wrinkling, roughness, laxity and pigmentation. In the present work, the impact of Biofield Energy (The Trivedi Effect®-Consciousness Energy Healing) Treatment on the herbomineral test formulation and cell medium (DMEM) was evaluated for skin health parameters. The test formulation was consisted of minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two parts. One part received the Biofield Energy Treatment by Jagdish Singh and was termed as the Biofield Treated (BT) sample, while other was denoted as the untreated (UT) samples. MTT assay showed that test formulation was found safe and nontoxic with greater than 75% cell viability against various tested concentrations. Cell proliferation data using BrdU method showed an improved cell proliferation by 149.13% and 118.80% at 8.75 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the untreated group. The collagen level was significantly increased by 28.14% and 44.45% at 1.25 and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The elastin level was increased by 10.38%, 14.66%, and 48.24% at 2.5, 1.25, and 0.625 µg/mL, respectively in the BT-DMEM + UT-Test formulation group, compared with the untreated group. Moreover, melanin synthesis was significantly inhibited by 5.93%, 1.43%, and 1.43% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.0625 µg/mL compared with the untreated group. However, melanin synthesis was decreased by 11.71% and 15.75% at 0.125 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively. Anti-wrinkling effects exhibited improved cell viability by 17.19% and 28.68% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively in HFF-1 cells compared with the untreated group. Wound healing activity using scratch assay showed a significantly improved healing rate upto 5% in the HFF-1 and HaCaT cells lines in the Biofield Energy Healing based test formulation. Overall, the data suggests that The Trivedi Effect® treated test formulation and DMEM has the capacity to improve the skin health and suggests its use in psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, and many more skin diseases.
Category: Chemistry

[14] viXra:1707.0080 [pdf] submitted on 2017-07-06 02:22:12

Skin Rejuvenating Effect of Consciousness Energy Healing Treatment Based Herbomineral Formulation

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, James Jeffery Peoples
Comments: 11 Pages.

Currently in today’s world, herbomineral based cosmetic formulations are continuously gaining popularity globally because they have a high safety profile and skin protection effects. The aim of this study was to evaluate the effects of a Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation and medium (DMEM) against different skin health parameters. The test formulation and DMEM were divided into two parts. One part of each received the Consciousness Energy Healing Treatment by James Jeffery Peoples and were termed as the Biofield Energy Treated samples, while the other samples were denoted as the untreated test items. MTT assay showed more than 70% viable cells, indicating that the test formulation was safe and nontoxic in all the tested concentrations in three cell lines. The percent cell proliferation by BrdU assay was increased by 251.30%, 373.36%, and 722.48% at 8.75, 17.5, and 35 µg/mL, respectively in the BT-DMEM + BT-Test formulation group compared to the UT-DMEM + UT-Test formulation group. Collagen synthesis was significantly (p≤0.001) increased by 32.96% in the UT-DMEM + BT-Test formulation group at 2.5 µg/mL with respect to the untreated group. Elastin was significantly (p≤0.001) increased by 69.49% and 17.13% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 10 µg/mL compared to the untreated group. The level of hyaluronic acid was increased remarkably by 14.78% in the BT-DMEM + UT-Test formulation group at 1.25 µg/mL compared to the untreated group. Melanin was suppressed significantly by 14.64% and 18.25% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 0.13 µg/mL compared to the untreated group. Skin cells protection against UV-B exposure revealed that the percent cell viability was increased by 4.23% in the BT-DMEM + BT-Test formulation group at 2.5 µg/mL compared to the untreated group. The wound healing activity by scratch assay showed significant wound closure and cell migration activity in the test formulation and DMEM group in both HFF-1 and HaCaT cells compared to the untreated group. Altogether, the data suggests that the Biofield Energy Treated DMEM and test formulation showed better responses compared to the untreated group. Overall, the Biofield Energy Treated test formulation could be developed as an effective cosmetic product to protect and treat various skin problems including infection, photosensitivity, cutis rhomboidalis nuchae, erythema, contact dermatitis, behcet's disease, blepharitis, boil, bowen's disease, skin aging, wrinkles and/or change in skin colour, etc.
Category: Chemistry

[13] viXra:1707.0063 [pdf] submitted on 2017-07-05 06:01:28

Impact of Consciousness Energy Healing Treatment on Herbomineral Formulation in Different Skin Health Parameters

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Deborah Lea Smith
Comments: 10 Pages.

The aim of this study was to evaluate the effect of the Consciousness Energy Healing based test formulation and cell medium (DMEM) against various skin health parameters. The test formulation and DMEM were divided into two parts. One part received the Consciousness Energy Healing Treatment (The Trivedi Effect®) by Deborah Lea Smith and termed as the Biofield Energy Treated sample, while other part denoted as untreated test item. The study was assessed for the measurement of collagen, elastin, hyaluronic acid, melanin synthesis, cell viability against UV-B induced stress, and wound healing activity. MTT assay showed that the test formulation was safe and nontoxic in three cell lines. Bromodeoxyuridine (BrdU) assay data showed that the percent cell proliferation was increased significantly by 22.69% and 34.99% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 8.75 µg/mL compared to the UT-DMEM + UT-Test formulation group. Collagen was increased by 15.12% and 15.26% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL compared to the untreated group. Elastin was significantly increased by 9.10%, 13.71%, and 12.41% in the UT-DMEM + BT-Test formulation, BT-DMEM + Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 5 µg/mL compared to the untreated group. Elastin was also increased significantly by 17.78% and 6.01% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 10 µg/mL compared to the untreated group. Hyaluronic acid was significantly increased by 3.90%, 5.37%, and 6.82% in the UT-DMEM + BT-Test formulation at 0.625, 1.25, and 2.5 µg/mL, respectively compared to the untreated group. Melanin level was reduced by 14.64% and 18.25% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 0.13 µg/mL compared to the untreated group. Skin cells protection was significantly increased by 21.32%, 23.81%, and 7.20% at 0.625, 1.25, and 2.5 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared to the untreated group. Wound healing assay exhibited significant wound closure and cell migration in all the tested groups compared to the untreated group. Overall, the Biofield Energy Treated test formulation and DMEM (The Trivedi Effect®) were found to be safe and can be developed as an effective cosmetic product to protect and treat the skin problems including infection, photosensitivity, acne, hives, chickenpox, eczema, rosacea, seborrheic dermatitis, psoriasis, erythema, contact dermatitis, skin aging, wrinkles, etc.
Category: Chemistry

[12] viXra:1707.0055 [pdf] submitted on 2017-07-05 01:05:04

Solid State Characterization of Withania Somnifera (Ashwagandha) Root Extract After Treatment with Consciousness Energy Healing

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak
Comments: 10 Pages.

Ashwagandha root extract is useful as an herbal medicine and nutraceuticals for the prevention and treatment of various diseases. The aim of the current study was to evaluate the influence of Consciousness Energy Healing Treatment (The Trivedi Effect®) on the physico-chemical, thermal and behavioral properties of ashwagandha root extract using powder X-ray diffraction (PXRD), particle size distribution analysis (PSD), Fourier transform infrared (FT-IR) spectrometry, UV-Vis spectroscopy, thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). Ashwagandha root extract was divided into two parts – one part was control without any Biofield Energy Treatment, and another part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as Biofield Energy Treated sample. The PXRD analysis concluded that both the control and treated samples were amorphous in nature. The particle size values at d10, d50, and d90 of the treated sample were significantly decreased by 36.78%, 15.18%, and 5.06%, respectively compared with the control sample. Likewise, the surface area of the treated sample was significantly increased by 85.14% compared to the control sample. FT-IR results showed a small impact of Consciousness Energy Healing Treatment on the phytoconstituents of ashwagandha root extract to reduce the force constant of O-H (str.) bond. UV-vis analysis revealed that the wavelength for the maximum absorbance (λmax) of both the samples was at 206.4 in methanol. TGA revealed the three steps of thermal degradation and the total weight loss was decreased by 0.73% in the treated sample compared to the control sample. Consequently, the maximum thermal degradation temperature was found at 272.53°C and 393.35°C for two broad peaks in the treated sample was increased by 0.05% and 0.08%, respectively compared to the control the sample (272.67°C and 393.66°C). The DSC analysis indicated that the evaporation temperature and latent heat of vaporization were lowered significantly by 4.98% and 35.67%, respectively in the treated sample compared with the control sample. The current outcomes suggested that the Energy of Consciousness Healing Treatment might have the amazing capacity to enhance the solubility, dissolution, absorption, bioavailability and thermal stability of ashwagandha root extract in the various form of pharmaceutical and nutraceutical formulation by modifying its particle size and surface area. Thus, the Biofield Energy Treated ashwagandha root extract might provide better therapeutic response against inflammatory diseases, immunological disorders, sexual disorders, arthritis, stress, cancer, ageing, diabetes, and other chronic infections.
Category: Chemistry

[11] viXra:1707.0054 [pdf] submitted on 2017-07-05 01:06:42

Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania Somnifera (Ashwagandha) Root Extract

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak
Comments: 10 Pages.

Withania somnifera (ashwagandha) root extract is a popular health supplement, with purported health benefits including prevention and treatment of various diseases, i.e. anxiety, stress, etc. The objective of this experiment was to evaluate the impact of Energy of Consciousness Healing Treatment (The Trivedi Effect®) on the characteristic properties of the phytoconstituents present in the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while another part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The liquid chromatographic data of the control and Biofield Energy Treated samples revealed that the retention time of the 25 chromatographic peaks remained same, whereas the peak area% i.e. the relative amount of the phytoconstituents was altered significantly. The peak area% of the treated ashwagandha root extract representing the phytoconstituents was significantly decreased in the range of 6.02% to 39.74% at Rt of 5.2, 5.3, 5.4, 5.6, 5.7, 6.9, 7.1, 7.3, 7.8, 7.9, 8.0, and 8.5 minutes compared to the control sample. On the contrary, the peak area% of the other phytoconstituents present in the treated sample was significantly increased in the range of 4.12% to 82.32% at Rt of 5.1, 6.4, 6.6, 6.8, 8.1, 8.2, 8.4, 8.6, 8.8, 8.9, 9.0, 9.1, and 9.2 minutes, respectively compared to the control sample. A total of 16 withanolides such as sitoindoside IX, viscosa lactone B, dihydrowithanolide D, withanolide A, withaferin A, ixocarpalactone A, withanolide sulfoxide, etc. were proposed from the molecular mass at m/z 605, 489, 473, 471, 505, and 992 at the retention times of 6.9, 7.1, 7.8, 8.2, 8.5, and 9.2 minutes with the help of LC-MS, GC-MS and NMR data of both the samples. Subsequently, the mass peak intensities of the treated sample were significantly changed in the range of -61.24% to 106.61% compared with the control sample at the same retention time. These findings suggest that Energy of Consciousness Healing Treatment could be advantageous for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of ashwagandha extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, arthritis, stress, cancer, diabetes, sexual disorders, aging, and other chronic infections.
Category: Chemistry

[10] viXra:1707.0053 [pdf] submitted on 2017-07-05 01:07:25

A Systematic Study of the Biofield Energy Healing Treatment on Physicochemical, Thermal, Structural, and Behavioral Properties of Magnesium Gluconate

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak
Comments: 11 Pages.

Magnesium gluconate is a pharmaceutical/nutraceutical compound used as a source of magnesium ion. The recent study described the impact of The Trivedi Effect®-Energy of Consciousness Healing Treatment on magnesium gluconate for the variation in physicochemical, structural, thermal and behavioral properties using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Magnesium gluconate was divided into two parts – one part was control without any Biofield Energy Treatment, while another part was treated with The Trivedi Effect®-Energy of Consciousness Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The PXRD analysis exhibited that the crystallite size of the treated sample was remarkably altered from -70% to 130% compared with the control sample. The average crystallite size was significantly decreased by 23.74% in the treated sample compared to the control sample. Biofield Energy Healing Treatment significantly reduced the particle size of magnesium gluconate at d10, d50, and d90 values by 12.15%, 8.98% and 15.35%, respectively compared to the control sample. The surface area analysis showed that surface area of the treated sample was significantly increased by 11.76% compared with the control sample. The FT-IR and UV-vis analysis displayed that structure of the magnesium gluconate persisted identical in both the treated and control samples. The TGA analysis exhibited four steps thermal degradation in both samples and the total weight loss of the Biofield Energy Treated sample was reduced by 0.19% compared with the control sample. The melting temperature of the Biofield Energy Treated sample (171.25ºC) was slightly (0.16%) higher from the control sample (170.97ºC). The latent heat of fusion was significantly decreased by 7.76% in the treated sample compared to the control sample. The TGA and DSC analysis revealed that the thermal stability of the treated sample was enhanced compared with the control sample. The current study revealed that The Trivedi Effect®-Energy of Consciousness Healing Treatment might produce a new polymorphic form of magnesium gluconate, which could be more soluble and bioavailable along with improved thermal stability compared with the untreated compound. The Biofield Treated sample could be more stable during manufacturing, delivery or storage conditions than the untreated sample. Hence, The Trivedi Effect® Treated magnesium gluconate would be very useful to design better nutraceutical/pharmaceutical formulations that might offer better therapeutic responses against inflammatory diseases, immunological disorders, stress, aging, and other chronic infections.
Category: Chemistry

[9] viXra:1707.0052 [pdf] submitted on 2017-07-05 01:09:38

Photo-Protective Effect of Biofield Energy Healing (The Trivedi Effect®) Treatment Based Herbomineral Formulation Against Various Skin Health Parameters

Authors: Aksana Hancharuk, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak
Comments: 11 Pages.

The current study aimed to evaluate the effect of Biofield Energy Healing (The Trivedi Effect®) based herbomineral formulation on various skin health parameters. The test formulation consisted of minerals (zinc, selenate, and molybdenum), L-ascorbic acid, along with Centella asiatica extract, and tetrahydrocurcumin (THC). The test formulation and DMEM were divided into two parts, one part of each was treated with the Biofield Energy Treatment by Aksana Hancharuk and denoted as treated group, while other part was coded as the untreated groups. Skin parameters against various cell lines such as HFF-1, HaCaT and B16-F10 were examined with the combination of Biofield Treated (BT) and untreated test formulation (UT) with DMEM. MTT assay result showed that the test formulation was found to be safe and nontoxic at tested concentrations. Human fibroblast cell proliferation using BrdU method showed a significant increased cell proliferation by 91.51% and 201.14% in different Biofield Energy Treated groups. UT-DMEM + BT-Test formulation group showed an increased level of collagen by 64.58% (2.5 µg/mL) and 55.55% (1.25 µg/mL), respectively compared with the untreated group. Similarly, elastin synthesis was increased in UT-DMEM + BT-Test formulation group by 21.96% and 11.42% at concentration 10 and 5 µg/mL, respectively compared to the untreated group. However, hyaluronic acid level was increased by 14.02% and 6.57% at concentrations 1.25 and 0.625 µg/mL, respectively in BT-DMEM + BT-Test formulation group compared with the untreated group. Moreover, inhibition of melanin synthesis was observed by 12.95% and 17.86% (0.125 µg/mL) in UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the untreated group in B16-F10 melanoma cell line. Anti-wrinkling activity in HFF-1 cells showed a notable cell viability in all the groups at various concentrations, i.e. in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation group by 15.68%, 36.47%, and 29.64%, respectively at 2.5 µg/mL compared with the untreated group. The significant wound healing activity was reported in scratch assay in HFF-1 and HaCaT cells due to the significantly higher cellular migration of fibroblast and keratinocytes. Therefore, Biofield Energy Treatment (The Trivedi Effect®) based test formulation and cell medium might be a suitable approach for the development for herbal cosmetics and formulations as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating action. It can improve the overall skin health care against many skin disorders such as Eczema, diaper rash, seborrheic dermatitis, chickenpox, measles, warts, acne, hives, ringworm, Rosacea, psoriasis, skin cancer, etc.
Category: Chemistry

[8] viXra:1707.0051 [pdf] submitted on 2017-07-05 01:10:26

Skin Photo-Protective and Anti-ageing Activity of Consciousness Energy Healing Treatment Based Herbomineral Formulation

Authors: Bonnie P. Hegarty-Diaz, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak
Comments: 11 Pages.

The objective of this study was to evaluate the impact of The Trivedi Effect®- Consciousness Energy Healing Treatment based herbomineral formulation on various skin parameters using HFF-1, HaCaT, and B16-F1 cell lines. The test formulation was composed of minerals (zinc, selenium, and molybdenum), and L-ascorbic acid along with the mixture of Centella asiatica extract and tetrahydrocurcumin (THC). Test formulation and DMEM were divided into two equal parts, one was treated with the Biofield Energy Treatment by Bonnie P. Hegarty-Diaz and denoted as treated, while other part was coded as untreated groups. MTT assay result showed that the test formulation was found safe and nontoxic at tested concentration 40 µg/mL. Fibroblast cell proliferation assay showed significant increased cell proliferation by 26.83% at 8.75 µg/mL in BT-DMEM + BT-Test formulation group compared to the untreated group. The level of collagen was significantly increased by 22.05% and 22.24% (at 2.5 µg/mL) in BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively. However, collagen amount was increased by 11.08% and 9.52% in BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 1.25 µg/mL, compared to untreated group. Similarly, elastin synthesis was increased in the BT-DMEM + UT-Test formulation group by 19.98%, 72.54% and 105.04% at the concentrations 10, 5, and 2.5 µg/mL, respectively compared to the untreated group. However, level of hyaluronic acid was increased by 5.19% at 0.625 µg/mL in UT-DMEM + BT-Test formulation group compared with the untreated group. Besides, melanin synthesis inhibition was found to be inhibited by 14.80% and 17.40% at 0.125 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the untreated group in B16-F10 melanoma cell line. Anti-wrinkling activity in HFF-1 cells showed improve cell viability in all the groups at various concentrations, i.e. in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation group by 10.69%, 6.9%3, and 11.86%, respectively at 0.625 µg/mL compared with the untreated group. In addition, wound healing scratch assay data suggest significantly higher cellular migration of fibroblast and keratinocytes cells in HFF-1 and HaCaT cells lines, respectively. In conclusion, Biofield Energy Healing (The Trivedi Effect®) based test formulation and DMEM could be beneficial against various skin disorders such psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections and can be significantly used as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating action.
Category: Chemistry

[7] viXra:1707.0029 [pdf] submitted on 2017-07-03 02:02:37

Assessment of the Consciousness Energy Healing Treated Withania Somnifera (Ashwagandha) Root Extract Using LC-MS, GC-MS, and NMR Spectroscopy

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak
Comments: 11 Pages.

Withania somnifera (Ashwagandha) root extract is an important herbal medicine. The current study was designed to investigate the impact of Consciousness Energy Healing Treatment (The Trivedi Effect®) on the characteristic properties of the phytoconstituents present in the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while another part was treated with the Consciousness Energy Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The LC-MS analysis revealed that the retention time (Rt) of the phytoconstituents remained same in both the control and treated samples, whereas the peak area% was significantly altered. The peak area% of the treated sample at Rt of 5.06, 5.24, 5.49, 5.68, 6.78, 6.87, 8.12, 8.22, 8.36, 8.55, 9.02, and 10.13 minutes were significantly increased in the range of 0.27% to 146.94% compared to the control sample. In addition, the peak area% of the treated sample at Rt of 5.29, 5.36, 6.40, 6.49, 6.56, 6.67, 7.08, 7.37, 7.76, 7.87, 8.02, 8.43, 8.85, 8.97, and 9.12 minutes were significantly decreased in the range of 1.83% to 23.53% compared with the control sample. A total of 18 withanolides such as sitoindoside IX, viscosa lactone B, 24, 25-dihydrowithanolide D, withanolide A, withanone, withaferin A, withanolide D, ixocarpalactone A, withanolide S, withanolide sulfoxide, etc. were proposed with their structure from the molecular mass at m/z 650, 489, 473, 471, 505, and 992 at retention times of 6.87, 7.10, 7.87, 8.22, 8.54, and 9.11 minutes with the help of GC-MS and NMR data of both the control and Biofield Energy Treated samples. The mass peak intensity values of the Biofield Energy Treated sample at the same retention time in was significantly decreased in the range of 3.99% to 52.28% compared with the control sample. These findings suggest that Energy of Consciousness Healing Treatment could be beneficial for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of ashwagandha root extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.
Category: Chemistry

[6] viXra:1707.0028 [pdf] submitted on 2017-07-03 02:04:40

Evaluation of the Physico-chemical, Thermal and Behavioral Properties of Ashwagandha Root Extract: Effects of Consciousness Energy Healing Treatment

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak
Comments: 11 Pages.

Withania somnifera (Ashwagandha) root extract has a broad range of pharmacological activities due to the presence of many biologically active metabolites. The current study was designed to evaluate the influence of The Trivedi Effect® - Consciousness Energy Healing Treatment (Biofield Energy Healing) on the physical, spectroscopic, thermal and behavioral properties of ashwagandha root extract using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Ashwagandha root extract was divided into two parts – one part was control without any Biofield Energy Treatment and another part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as Biofield Energy Treated sample. The PXRD analysis exhibited that both the control and treated samples were amorphous in nature. The particle size values at d10, d50, and d90 of the treated sample were increased significantly by 8.69%, 7.32%, and 6.50%, respectively compared with the control sample. Similarly, the surface area of the treated sample was significantly decreased by 12.69% compared to the control sample. The FT-IR analysis indicated no significant alteration of the force constant for the functional groups in the treated sample compared to the control sample. The UV-vis analysis revealed that the wavelength for the maximum absorbance of the control and treated samples were at 207.5 and 205.2 nm, respectively. The TGA revealed the two major thermal degradation steps and the total weight loss was decreased by 0.86% in the treated sample compared with the control sample. Consequently, the maximum thermal degradation temperature at 273.53°C and 389.39°C for two broad peaks in treated sample was increased by 0.78% and 0.31%, respectively compared to the control the sample (271.40°C and 388.48°C). The DSC analysis indicated that the evaporation temperature was significantly increased by 10.95% in the Biofield Energy Treated sample with a 5.34% reduced the latent heat of vaporization compared with the control sample. The overall thermal analysis indicated that the treated sample was thermally more stable as compared to the control sample. The Trivedi Effect® - Energy of Consciousness Healing Treatment might lead to have better powder flowability and long-term storage stability compared with the control sample. Thus, the treated ashwagandha root extract might provide better therapeutic response against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, ageing, and other chronic infections.
Category: Chemistry

[5] viXra:1707.0027 [pdf] submitted on 2017-07-03 02:05:10

LC-MS, GC-MS, and NMR Spectroscopy Based Evaluation of the Energy of Consciousness Healing Treated Withania Somnifera (Ashwagandha) Root Extract

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak
Comments: 9 Pages.

Withania somnifera root extract which contains withanolides as major active constituent and it is used traditionally for the prevention and treatment of several diseases. The objective of the current study was to investigate the impact of The Trivedi Effect® - Consciousness Energy Healing Treatment on the structural properties of the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while other part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The LC-MS analysis revealed that the retention time of the phytoconstituents remained same in the control and Biofield Energy Treated samples, whereas the peak area% i.e. the relative amount of the phytoconstituents at respective retention time was significantly altered. The peak area% at Rt of 5.6, 6.8, 6.9, 7.2, 7.9, 8.4, 8.5, 8.6, and 9.2 minutes of the treated sample were increased significantly in the range of 1.46% to 253.06% compared to the control sample. In the contrary, the peak area% of the treated sample at Rt of 6.4 and 8.2 minutes were significantly decreased by 12.72% and 17.35%, respectively with respect to the control sample. A total of 16 withanolides such as withanoside IV, coagulin Q, viscosa lactone B, dihydrowithanolide D, withanolide A, withaferin A, withanone, withanolide D, ixocarpalactone A, withanolide sulfoxide, withanolide B, etc. were proposed with their structure from the molecular mass at m/z 783, 621, 489, 473, 767, 471, 505, and 992 at retention times of 6.4, 6.8, 7.2, 7.9, 8.2, 8.4, and 9.1 minutes, respectively with the help of LC-MS, GC-MS and NMR data of both the control and Biofield Energy Treated samples. The mass peak intensities of the Biofield Energy Treated sample were significantly changed in the range of -81.36% to 1720.90% compared with the control sample at the same retention time. These findings suggest that The Trivedi Effect® - Consciousness Energy Healing Treatment could be beneficial for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of W. somnifera extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging, and other chronic infections.
Category: Chemistry

[4] viXra:1707.0025 [pdf] submitted on 2017-07-03 02:05:44

Consciousness Energy Healing Treatment on Withania Somnifera Root Extract and Its Effects on the Physical, Spectroscopic, Thermal and Behavioral Properties

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak
Comments: 10 Pages.

Withania somnifera (Ashwagandha) is useful as an herbal medicine and nutraceuticals for the prevention and treatment of anxiety, insomnia, tuberculosis, tumors, menstrual problems, etc. The objective of the current study was to evaluate the influence of The Trivedi Effect® - Consciousness Energy Healing Treatment on the physical, spectroscopic, thermal and behavioral properties of ashwagandha root extract using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Ashwagandha root extract was divided into two parts – one part was control without any Biofield Energy Treatment and another part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as Biofield Energy Treated sample. The PXRD analysis demonstrated that both the control and treated samples were amorphous in nature. The particle size values at d10, d50, and d90 of the treated sample were decreased significantly by 18.88%, 8.60%, and 4.00%, respectively compared with the control sample. Similarly, the surface area of the treated sample was significantly increased by 102.63% compared to the control sample. FT-IR results suggest that there was a small impact of Biofield Energy Treatment on ashwagandha root extract at the atomic level to reduce the force constant of O-H (str.) bond. UV-vis analysis revealed that the wavelength for the maximum absorbance of the control and treated samples were at 207.4 and 207.7 nm, respectively. TGA revealed the three steps of thermal degradation steps and the total weight loss was decreased by 0.13% in the treated sample compared with the control sample. Consequently, the maximum thermal degradation temperature at 272.73°C and 393.14°C for two broad peaks in treated sample was increased by 1.13% and 0.85%, respectively compared to the control the sample (269.68°C and 389.83°C). DSC analysis indicated that the evaporation temperature and latent heat of vaporization was increased by 1.35% and 5.52%, respectively in the treated sample compared with the control sample. The current findings suggested that The Trivedi Effect® - Energy of Consciousness Healing Treatment might have the astounding capacity to enhance the solubility, absorption, dissolution, bioavailability and thermal stability of ashwagandha root extract in various form of pharmaceutical and nutraceutical formulation by modifying its particle size and surface area. Thus, the treated ashwagandha root extract might provide better therapeutic response against inflammatory diseases, immunological disorders, arthritis, sexual disorders, stress, cancer, diabetes, ageing, and other chronic infections.
Category: Chemistry

[3] viXra:1707.0024 [pdf] submitted on 2017-07-03 02:06:14

Study of the Consciousness Energy Healing Treated Ashwagandha Root Extract by LC-MS, GC-MS, and NMR Spectroscopy

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak
Comments: 10 Pages.

Withania somnifera (ashwagandha) contains many biologically active constituents used for the prevention and treatment of various diseases. The aim of the current study was to evaluate the impact of Energy of Consciousness Healing Treatment (The Trivedi Effect®) on the characteristic properties of the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while another part was treated with the Energy of Consciousness Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. LC-MS data revealed that the retention time of the phytoconstituents remained same in the control and Biofield Energy Treated samples, whereas the peak area% i.e. the relative amount of the phytoconstituents was significantly altered. The peak area% at Rt of 5.1, 5.7, 6.4, 6.6, 6.9, 7.1, 7.4, 7.8, 7.9, 8.0, 8.2, 8.5, and 8.9 minutes of the treated sample were increased in the range of 0.92% to 14.67% compared to the control sample. On the contrary, the peak area% of the treated sample at Rt of 5.2, 5.3, 5.4, 5.6, 6.8, 8.1, 8.4, 8.6, 9.0, 9.1, and 9.2 minutes were decreased by 1.59% to 36.69% with respect to the control sample. A total of 14 withanolides such as sitoindoside IX, dihydrowithanolide D, withanolide A, withaferin A, ixocarpalactone A, withanolide S, withanolide sulfoxide, etc. were proposed with their structure from the molecular mass at m/z 605, 489, 473, 471, 505, and 992 at retention times of 6.9, 7.1, 7.9, 8.2, 8.5, and 9.3 minutes with the help of LC-MS, GC-MS and NMR data of both the control and Biofield Energy Treated samples. Consequently, the mass peak intensities of the treated sample were significantly changed in the range of -36.67% to 106% compared with the control sample at the same retention time. These findings suggest that Energy of Consciousness Healing Treatment could be advantageous for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of ashwagandha extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, arthritis, stress, cancer, diabetes, sexual disorders, aging, and other chronic infections.
Category: Chemistry

[2] viXra:1707.0008 [pdf] submitted on 2017-07-01 08:25:08

Standing Waves

Authors: John Atwell Moody
Comments: Pages.

Contents

Part I
O(j) is not fully equivariant if j is odd
Representations of SO3
Eigenfunctions of the Laplacian
Attempts to explain the periodic table
Attempts to explain atomic spectra

Part II
Three corrections to the fine structure
A discussion of perturbation theory
Half-integer values of l – first explanation
Generalities about the Hamiltonian formulation
Schroedinger’s function W
Half-integer values of l – other explanations
The atom
Schroedinger’s choice of solutions
Residues
The canonical divisor and j quantum number
Intuitive description of polarization
Relation between smooth and holomorphic Laplacian operators
A coincidence about the periodic table
Linear systems of wave forms
Hodge theory
The Levi-Civita action is not Lagrangian for its Riemann metric
Foundations
Unification of the various coupling schemes
Discussion of the problem of non-convergent integrals
The ethics of science
First concepts of the Lamb shift
A question about Pauli exclusion
The notion of probability
Concluding remark
False fine structure
Electron spin and Pauli exclusion
It was not the right conclusion

Category: Chemistry

[1] viXra:1707.0001 [pdf] submitted on 2017-07-01 04:51:48

Atomistic Description of the Hydration Process of Graphene Oxide

Authors: Antenor Neto, Vitaly V. Chaban, Eudes E. Fileti
Comments: 18 Pages.

Hydration of graphene oxide (GO) is an important process for most of areas planning to use it in practical applications. This process is described in this work by molecular dynamics atomistic simulations for seven different models for GO at different levels of oxygenation. Two charge models for GO were considered: a simplified one, where sp2 carbons were treated as LJ uncharged sites and another with charges at all sites obtained by the CHELPG scheme. We observed that the structural properties suffer little or no effect in relation to the charge model, on the other hand the energetics is much more sensitive. Our model employing CHELPG charges shows that the simplified model tends to overestimate the GO/water interaction energy. For all of the investigated systems, hydration free energy values are in the range of -5 to -45 kJ mol-1 indicating that hydration is a favorable process for all investigated systems. The results presented here is relevant in the context of several applications, such as the use of GOs as electrodes in supercapacitors or inhibitors in processes involving biological molecules.
Category: Chemistry