Biochemistry

1905 Submissions

[6] viXra:1905.0535 [pdf] submitted on 2019-05-29 04:16:15

Impact of Consciousness Energy Healing Treatment on the Physicochemical and Thermal Properties of an Anticancer Drug 6-Mercaptopurine

Authors: Gopal Nayak, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Snehasis Jana
Comments: 9 Pages.

Mercaptopurine is an antineoplastic chemotherapy drug is used for the treatment of cancer, Crohn's disease, ulcerative colitis, autoimmune diseases, etc. In this scientific study, the impact of the Trivedi Effect®-Consciousness Energy Healing Treatment on the physicochemical and thermal properties of 6-mercaptopurine was evaluated using the modern analytical technique. The test sample 6-Mercaptopurine powder was divided and named into control and treated sample. The control sample did not receive the Biofield Energy Treatment; whereas, the treated sample received the Biofield Energy Treatment remotely by a well-known Biofield Energy Healer, Gopal Nayak. The particle size values in the treated 6-mercaptopurine sample were significantly decreased by 10.18% (d10), 10.59% (d50), 7.62% (d90), and 9.17% {D(4,3)}; thus, the specific surface area was significantly increased by 10.28% compared to the control sample. The powder XRD peak intensities and crystallite sizes of the treated 6-mercaptopurine were significantly altered ranging from -43.40% to 23.33% and -37.09% to 136.67%, respectively; whereas the average crystallite size was significantly decreased by 11.04% compared with the control sample. The latent heat of evaporation and latent heat of fusion of the treated 6-mercaptopurine were significantly increased by 11.81% and 14.97% compared with the control sample. The total residue amount was significantly increased by 6.44% in the treated sample compared with the control sample. The maximum thermal degradation temperature of the 1st, 2nd, and 3rd peaks of the treated sample was altered by 1.55%, 0.18%, and -1.29% compared with the control sample. It was concluded that the Trivedi Effect®-Consciousness Energy Healing Treatment might have generated a new polymorphic form of 6-mercaptopurine which would offer better solubility, absorption, and bioavailability compared with the control sample. The Biofield Energy Treated 6-mercaptopurine would be more efficacious against acute lymphocytic leukemia, chronic myeloid leukemia, Crohn's disease, ulcerative colitis, and autoimmune diseases.
Category: Biochemistry

[5] viXra:1905.0516 [pdf] submitted on 2019-05-28 05:36:36

Mahendra@trivedisrl.com

Authors: Mahendra Kumar Trivedi, Snehasis Jana
Comments: 7 Pages.

The present study was performed to investigate the Hepatoprotective potential of the Biofield Energy (The Trivedi Effect®) Treated test item, Dulbecco’s Modified Eagle Medium (DMEM) in HepG2 cells. The test item was distributed into two parts. One part received Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Mahendra Kumar Trivedi and was labeled as the Biofield Energy Treated DMEM group and the other part referred as the untreated DMEM group, where no Biofield Treatment was provided. Results showed that more than 97% cell viability of the test items were observed by MTT assay, which indicated a safe and nontoxic nature of the test items. The Biofield Treated DMEM showed significant (p≤0.001) protection of cells by 10% against oxidative stress induced by t-BHP, while the untreated DMEM group showed 0.4% protection. The level of interleukin-8 (IL-8) was significantly (p≤0.01) reduced by 31.57% in Biofield Treated DMEM than untreated DMEM. The level of ALT enzyme activity was significantly (p≤0.001) reduced by 66% in Biofield Treated DMEM compared to untreated DMEM. Cholesterol level was significantly (p≤0.001) reduced by 46.23% in Biofield Treated DMEM than untreated DMEM. Besides, Biofield Treated DMEM group showed 51.18% increased the level of albumin compared to untreated DMEM group. Overall, results demonstrated that Biofield Treatment significantly protect the liver hepatocytes against oxidative stress. Therefore, Consciousness Energy Healing (The Trivedi Effect®) Treatment might be useful as a hepatoprotectant against different types of liver injuries like cirrhosis, alcohol abuse, hemochromatosis, Wilson’s disease, Gilbert’s disease, cholangiocarcinoma, steatosis, Budd-Chiari syndrome, etc.
Category: Biochemistry

[4] viXra:1905.0484 [pdf] submitted on 2019-05-25 05:37:37

In vitro Assessment of the Biofield Treated Test Item on Cardiac Function Using Rat Cardiomyocytes Cell Line (H9c2) via Multiparametric Analysis

Authors: Mahendra Kumar Trivedi, Snehasis Jana
Comments: 12 Pages.

Introduction: Heart disorders are the major concern of population health worldwide. According to WHO estimates 2018, 17.9 million peoples were died due to cardiovascular disorders. Aim: The aim of this study was to investigate the cardioprotective activity of Biofield Energy Treated test item, Dulbecco's Modified Eagle Medium (DMEM) using rat cardiomyocytes (H9c2). Methods: The test item (DMEM) was divided into three parts, first part received one-time Biofield Energy Treatment by a renowned Biofield Energy Healer, Mahendra Kumar Trivedi and was labeled as the one-time Biofield Energy Treated (BT-I) DMEM, while second part received the two-times Biofield Energy Treatment and is denoted as BT-II DMEM. The third part did not receive any treatment and defined as the untreated DMEM group. Results: Cell viability of the test samples by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay showed 89.03% and 98.49% in the BT-I and BT-II groups, respectively suggested a nontoxic and safe in nature of the tested test item. The BT-I group showed 16.01% restoration of cell viability. The level of lactate dehydrogenase (LDH) was significantly inhibited by 50.37% and 49.35% in the BT-I and BT-II groups, respectively compared to the untreated DMEM group. Moreover, percent protection of creatine kinase-myocardial band (CK-MB) by 49.48% and 59.79% in the BT-I and BT-II groups, respectively, compared to the untreated DMEM group. Reactive oxygen species (ROS) level in terms of mean fluorescence unit (FU) was reduced by 6.64% in the BT-I group than untreated DMEM. Besides, BT-I and BT-II groups significantly increased the level of % apoptotic cells by 63.16% and 97.37% (p≤0.05), respectively than untreated DMEM. Conclusion: Allover, results envisaged that Biofield Treatment significantly improved different cardiac parameters. Thus, Biofield Energy Treatment (The Trivedi Effect®) could be utilized as a cardio-protectant against several cardiac disorders such as coronary artery disease, heart attack, arrhythmias, heart failure, congenital heart disease, cardiomyopathy, etc.
Category: Biochemistry

[3] viXra:1905.0483 [pdf] submitted on 2019-05-25 05:38:21

Biofield Energy Treatment: Physicochemical and Thermal Characterization of L-Tryptophan

Authors: Dahryn Trivedi, Mahendra Kumar Trivedi, Alice Branton, Gopal Nayak, Snehasis Jana
Comments: 7 Pages.

L-tryptophan is an essential amino acid that needs to be supplemented through protein-rich diet and it helps in growth and nitrogen balance in the humans. The aim of this study is to evaluate the effect of the Trivedi Effect®-Consciousness Energy Healing Treatment on the L-tryptophan in terms of its physicochemical and thermal properties by using modern analytical techniques. The method involved dividing the test sample into two parts and providing the Consciousness Energy Healing Treatment to one part (named as Biofield Energy Treated sample), remotely by a renowned Biofield Energy Healer, Dahryn Trivedi; while the other part didn’t receive any treatment called a control sample. The particle size values of the treated sample was increased by 4.77% (d10), 6.37% (d50), 6.72% (d90), and 6.49% {D(4,3)}; thus, the specific surface area was increased by 4.35% compared to the control sample. The PXRD peak intensities and crystal-lite sizes of the treated sample were significantly altered ranging from -82.69% to 30.54% and -17.15% to 179.73%, respectively, in comparison to the control sample. The total weight loss of the treated sample was significantly reduced by 11.60% during the heating process that resulted in a significant increase in the residual amount of the sample by 1093.33% as compared to the control sample. The melting temperature and the latent heat of fusion of the treated sample were significantly increased by 1.12% and 6.79%, respectively, compared to the untreated sample. Hence, the L-tryptophan might form a new polymorph after the Trivedi Effect®-Consciousness Energy Healing Treatment that may possess better solubility and bioavailability profile along with improved thermal stability compared with the untreated sample. Thus, the Trivedi Effect®-Consciousness Energy Healing Treatment could be considered as a novel approach for designing the formulations with improved drug profile and efficacy. The treated L-tryptophan would be more useful for the treatment of mental health disorders, premenstrual dysphoric disorder (PMDD), attention deficit-hyperactivity disorder (ADHD), obsessive-compulsive disorder, depression, and bipolar disorder, etc.
Category: Biochemistry

[2] viXra:1905.0432 [pdf] submitted on 2019-05-22 23:35:05

Influence of the Biofield Energy Therapy on the Physico-Chemical and Thermal Properties of Sulfamethoxazole

Authors: Dahryn Trivedi, Mahendra Kumar Trivedi, Alice Branton, Gopal Nayak, Snehasis Jana
Comments: 7 Pages.

Sulfamethoxazole is an antibiotic, which inhibits the bacteria by interfering in its folic acid synthesis mechanism. The objective of this study was to analyze the Trivedi Effect®-Consciousness Energy Healing Treatment on the phys-icochemical and thermal properties of sulfamethoxazole by using modern analytical techniques. The sulfamethoxaz-ole sample was classified as control and treated samples. The control sample did not receive the Biofield Treatment, while the treated sample received the Biofield Treatment remotely by a renowned Biofield Energy Healer, Dahryn Trivedi. The particle sizes of the treated sample were significantly reduced by 7.99% (d10), 3.05% (d50), 3.80% (d90), and 3.03%{D(4,3)}; whereas, the specific surface area of the particles was increased by 5.19% in the treated sample compared to the control sample. The PXRD peak intensities and crystallite sizes were altered ranging from -70.86% to 52.36% and -6.01% to 75.34%, respectively; whereas the average crystallite size was significantly increased by 27.28% in the treated sulfamethoxazole compared with the control sample. The sample residual amount and maximum thermal degradation temperature were increased by 2.13% and 4.21%, respectively, in the treated sample compared to the con-trol sample. The decomposition temperature and latent heat of decomposition were increased by 3.26% and 19.36%, respectively, in the treated sample compared to the untreated sample. The Biofield Treated sulfamethoxazole might con-vert to a novel polymorph of the drug, with reduced particle size, increased surface area, and improved thermal stability. This can be helpful in improving the solubility, bioavailability, and stability of the sulfamethoxazole and would be more efficacious for the treatment of infections in the ear, urinary tract infections, traveler’s diarrhoea, bronchitis, shigellosis, and pneumonia, etc.
Category: Biochemistry

[1] viXra:1905.0431 [pdf] submitted on 2019-05-22 23:35:53

Impact of Biofield Energy Treated DMEM on Mitochondrial Biogenesis Using Myoblast Cell Line, C2C12: Implications for Metabolic Disease

Authors: Mahendra Kumar Trivedi, Snehasis Jana
Comments: 7 Pages.

Mitochondria play an important role in the body, and its dysfunction lead to the defective cellular energy production results to heterogeneous group of disorders such as early aging, type-2 diabetes, Alzheimer’s disease, and many more metabolic diseases. The study was aimed to examine the effect of Consciousness Energy Healing based DMEM on myoblasts cells (C2C12) for mitochondrial mass content. The test item, DMEM was divided into three parts. First part did not receive any sort of treatment and defined as the untreated DMEM group. The second and third portions were treated with the one-time and two-times Biofield Energy Treatment by a renowned Biofield Energy Healer, Mahendra Kumar Trivedi and coded as the one-time Biofield Energy Treated DMEM (BT-I) and two-times Biofield Energy Treated DMEM (BT-II) groups, respectively. Cell viability of the test items by MTT assay showed more than 72% viable cells, which suggested that the test items were nontoxic and safe in nature. Moreover, the mitochondrial mass content in terms of fluorescence unit (FU) was significantly (p≤0.05) increased by 43.11% and 64.16% in the BT-I and BT-II groups, respectively in C2C12 cells compared to the untreated DMEM group. Overall, the experimental data suggested that two-times treated DMEM (BT-II) was found more significant improvement in mitochondrial mass content compared with the one-time treated DMEM (BT-I) group and results in better respiratory capacity. Thus, data envisaged that the Biofield Energy Treated DMEM has a potential to improve respiratory activity, which could be used against various metabolic diseases, such as insulin resistance, type-2 diabetes, and cardiovascular diseases, etc.
Category: Biochemistry