Skin reflects the correlation between the overall inner-health and aging status, which is the complex biological process influenced by the various factors. The present work evaluated the impact of Biofield Energy (The Trivedi Effect®-Consciousness Energy Healing) Treatment on the test formulation and cell medium (DMEM) for various skin health parameters using HFF-1, HaCaT, and B16-F10 cells. The test formulation was consisted of seven ingredients viz. zinc chloride, ferrous sulfate, copper chloride, magnesium gluconate, pyridoxine HCl, vitamin B12, and vitamin D3. The test formulation and DMEM media were divided into two parts. One part received the Biofield Energy Treatment by a renowned Biofield Energy Healer, Alice Branton and defined as the Biofield Treated (BT) sample, while other was denoted as the Untreated (UT) samples. MTT assay using test formulation showed greater than 76% cell viability, suggested safe and nontoxic profile at tested concentrations. Cell proliferation data using BrdU method showed an improved cell proliferation by 127.92% and 121.20% at 50 µg/mL in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively compared with the untreated group. The collagen level was significantly increased by 13.08%, and 15.98% at 50 and 100 µg/mL, respectively in the UT-DMEM + BT-Test formulation group compared with the untreated group. The level of elastin was increased by 28.09% at 50 µg/mL in the UT-DMEM + BT-Test formulation group, compared with the untreated group. Hyaluronic acid level was significantly (p≤0.001) increased by 86.10% and 35.14% at 10 and 50 µg/mL, respectively in the BT-DMEM + BT-Test formulation group as compared with the untreated group. Moreover, melanin synthesis was significantly inhibited by 16.96% at 10 µg/mL in the UT-DMEM + BT-Test formulation group compared with the untreated group. Anti-wrinkling effect exhibited improved cell viability by 65.63% and 69.95% at 1 and 10 µg/mL, respectively in the BT-DMEM + BT-Test formulation group compared with the untreated group. In vitro wound healing activity using scratch assay significantly improved healing rate and cell migration upto 58.55% in the HFF-1 cells in the UT-DMEM + BT-Test formulation group, while upto 231.25% in HaCaT cells lines in UT-DMEM + BT-Test formulation group compared with the untreated group. In conclusion, The Trivedi Effect® based test formulation and DMEM have the significant capacity to improve overall skin health, reversal of aging process and suggests its use in psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, and many more skin diseases.
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[v1] 2019-08-08 06:36:44
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