Biochemistry

   

Effect of Different Polarity Solvents on Total Phenols and Flavonoids Content, and in-Vitro Antioxidant Properties of Flowers Extract from Aurea Helianthus

Authors: Hyon-il Ri1, Un-hak Pak1, Jong-hyok Yang1, Kwen-il Son1, Yu-il Kim1, Kyong-chol Son1, Chol-jin Ri1, Myong-su Kang2, Tae-mun Kim2

The total phenols and flavonoids content of different polar solvent extracts from Aurea Helianthus flowers, and their antioxidant activity were determined. The ethanol extract of Aurea Helianthus flowers were suspended in water and fractionated using different polar solvents; hexane, chloroform, ethyl acetate, butanol and water. The parameters of each extract mentioned above were determined using Folin-Ciocalteu reagent (FCR) method, AlCl3 colorimetry method, Ferric reducing ability of plasma (FRAP) assay, total antioxidant activity (TAA) assay and DPPH radical scavenging assay. The highest total phenols content (516.21 mg GAE/g) and flavonoids content (326.06 mg QCE /g)were obtained in ethyl acetate extract, the correlation between TPC and TFC assay was found to be 0.967. All polar solvent extracts of Aurea Helianthus flowers showed significant antioxidant effects, the highest inhibition was obtained in ethyl acetate and chloroform extracts and the lowest inhibition in the water extract. There is a good correlation of total phenols and flavonoids content with antioxidant activity. This work indicated that the polar solvent extracts of Aurea Helianthus flowers contain high phenols and flavonoids content, and exhibited antioxidant activities in vitro, therefore, could be candidates for use as natural antioxidant.

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[v1] 2019-06-20 18:25:03

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