A simple, selective, accurate, and economical reverse phase high performance liquid chromatography (RP-HPLC) was developed for estimation of nicergoline in pharmaceutical formulations. Chromatographic separation achieved isocratically on a C18column (ODS, C18, 5μ, 250×4.6 mm i.d.) with mobile phase containing methanol, acetonitrile and 1.0 % ortho phosphoric acid in the ratio 80:18:2 v/v/v. The flow rate was 1.0mL/min and effluent was monitored at 265nm. The retention time was 3.128min. The method was validated in terms of linearity, accuracy and precision. The linearity curve was found to be linear over 1.0 - 6.0 μg/mL. The limit of detection and limit of quantification were found to be 0.3 μg /ml and 0.9 μg /ml respectively. The proposed method was successfully used to determine the drug content of marketed formulations.
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[v1] 2019-05-19 06:04:47
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