Authors: George Rajna
Multiphoton fluorescence microscopy, in which a fluorophore absorbs more than one photon and emits light at a shorter wavelength than the excitation source, can be used to create 3D tissue images at depths of 1 mm or more. [23] Researchers at the University of Twente have designed a tiny needle in which micro-channels can be used for extracting small liquid samples from a local area of the brain. [22] The ability to grow large protein crystals is the single biggest bottleneck that limits the use of neutron protein crystallography in structural biology. [21]
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