Biochemistry

   

Lab Report

Authors: Nilantha Gamalathge

Abstract Bifidobacteria Identification is important in medical and pharmaceutical industry. The molecular analytic technique such as PCR gel electrophoresis and MALDI –TOF-MS are high sensitive and useful technique in identifying Bifidobacteria. This practical was planned to identification of Bifidobacteria by both method. PCR method is based on denaturing, annealing and extension of target DNA in the bacteria in vitro with other requirements like taq polymerase enzyme, DNA primers, Mg etc with temperature control. MALDI –TOF-MS is based on analyet Matrix co crystallization and firing of laser beam get ionization and detection time of flight by mass spectrometry Un known 3 cutler plats (S1, S2,S3) were used for both methods and correctly identified up to genus level by PCR agarose gel electrophoresis. MALDI TOF MS was done in direct application and formic acid extraction method. Both ways identified up to bifid bacteria species and lactobacillus. Both methods were equally highly sensitive to identify Bifidobacteria. Identification of species was highly accurate MALDY TOF –MS method. But both methods have done with limited sample size. Both methods are complex, high cost and need high technical knowledge. MALDI method results depend on software apart from other steps. PCR can improve to identify Bifid bacteria up to species level with the use of species-level primers.MALDI method with formic acid exaction can improve sensitivity with the purification of the protein. (PCR-polymerase Chain Reaction.MALDI-TOF-MS- Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry).

Comments: 19 Pages.

Download: PDF

Submission history

[v1] 2018-08-04 02:48:56

Unique-IP document downloads: 5 times

Vixra.org is a pre-print repository rather than a journal. Articles hosted may not yet have been verified by peer-review and should be treated as preliminary. In particular, anything that appears to include financial or legal advice or proposed medical treatments should be treated with due caution. Vixra.org will not be responsible for any consequences of actions that result from any form of use of any documents on this website.

Add your own feedback and questions here:
You are equally welcome to be positive or negative about any paper but please be polite. If you are being critical you must mention at least one specific error, otherwise your comment will be deleted as unhelpful.

comments powered by Disqus