Analytical Comparison Between Microhematocrit and Automated Methods for Packed Cell Volume (PCV) Determination

Authors: Audu I. Stephen, Simon T. Ubwa, Ogbene G. Igbum, Stephen S. Hati, NwannadiI.Alex

Analytical methods comparison for the determination of packed cell volume(PCV) are essential in clinical laboratory practice as it improves the quality of health care through accurate and reliable clinical decision making from diagnostic results of suitable alternatives. Comparison is necessary because each method is expected to serve as a quality control measure for the other. This study was done to assess the analytical performance between the Microhematocrit and automated methods for PCV determination. In this study carried out at the heamatology laboratory of the Benue State University Teaching Hospital, Makurdi, Nigeria, the Microhematocrit method determined by using the HC 702, (ApelCo. Ltd, Korea) was compared with the Automated hematology Analyzer method (KX-21N sysmex, USA) using paired data of blood samples analyzed respectively from 206 patients in the hospital. Data analysis was performed using Analyse-it ® Version 4.6 method validation software. The results showed that similar overall mean values of PCV were obtained by both microhematocrit (34.5±7.3%) and automated (34.3±6.8%) methods. Result of t-test analysis was not statistically significant (p= 0.135) between the overall measurements by both methods.

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[v1] 2017-09-25 05:26:15

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