Authors: X. Michael Liu
Size Exclusion Chromatography (SEC) or High Performance Size Exclusion Chromatography (HPSEC) is the most broadly accepted method for the determination of molecular weights and molecular weight distributions of macromolecules. It is a special type of liquid chromatography that separates molecules based on molecular sizes or hydrodynamic volumes and not according to partition or affinities toward the stationary phases. HPSEC is also called Gel Permeation Chromatography (GPC) in polymer science and gel filtration chromatography (GFC) in the biological sciences. Conventional HPSEC equipped with a concentration detector (i.e. refractive index, UV-vis, or photodiode array) acquires molecular weights and molecular weight distributions of analytes according to the elution volumes or a peak position calibration using a series of standards of known molecular weights and chemistry. Therefore, the obtained molecular weights and molecular weight distributions by conventional HPSEC are the relative ones. The advantages of conventional SEC are high robustness, excellent reproducibility, and ease of transfer between laboratories. You can submit your Manuscripts at: https://symbiosisonlinepublishing.com/submitManuscript.php
Comments: 1 Page.
[v1] 2017-08-28 02:22:23
Unique-IP document downloads: 9 times
Vixra.org is a pre-print repository rather than a journal. Articles hosted may not yet have been verified by peer-review and should be treated as preliminary. In particular, anything that appears to include financial or legal advice or proposed medical treatments should be treated with due caution. Vixra.org will not be responsible for any consequences of actions that result from any form of use of any documents on this website.
Add your own feedback and questions here:
You are equally welcome to be positive or negative about any paper but please be polite. If you are being critical you must mention at least one specific error, otherwise your comment will be deleted as unhelpful.