Biochemistry

   

G-CSF Administration Accelerates Cutaneous Wound Healing Accompanied With Increased Pro-Hyp Production In db/db Mice

Authors: Shiro Jimi, Kenji Sato, Masahiko kimura, Junji Suzumiya, Shuuji Hara, Francesco De Francesco, Hiroyuki Ohjimi

Objective: Impaired wound healing in diabetic patients is a clinical concern. However, exacerbation factors in diabetic wounds are still not clear. Inflammatory cell infiltrates after skin wounding and subsequent healing responses were investigated using diabetic mice. Methods: Granulocyte colony-stimulating factor (G-CSF), neutrophil infiltration and peptides from degraded collagen in wounded tissue were examined using db/db and wild-type mice. Results: The collagen peptides Pro-Hyp and Hyp-Gly in wounded tissue were quantified. G-CSF was transiently secreted from wounded tissue immediately after excision and then appeared in peripheral blood. G-CSF levels were significantly lower in db/ db mice than in wild-type mice, and neutrophil infiltration into the granulation tissue was lower in db/db mice. In wound tissue, only Pro-Hyp increased during the 7-day study, and Pro-Hyp levels were significantly lower in db/db mice. Wound closure was severely impaired in db/db mice. However, topical recombinant human G-CSF (rhG-CSF) administration accelerated healing, accompanied with increased neutrophil infiltration and Pro-Hyp production. Conclusion: The results show that decreased G-CSF secretion in wound tissue may trigger delayed healing in diabetic mice and that topical rhG-CSF administration increased Pro-Hyp production and accelerated healing. Therefore, G-CSF-induced Pro-Hyp may play an important role in wound healing.

Comments: 9 Pages.

Download: PDF

Submission history

[v1] 2017-08-23 06:06:21

Unique-IP document downloads: 17 times

Vixra.org is a pre-print repository rather than a journal. Articles hosted may not yet have been verified by peer-review and should be treated as preliminary. In particular, anything that appears to include financial or legal advice or proposed medical treatments should be treated with due caution. Vixra.org will not be responsible for any consequences of actions that result from any form of use of any documents on this website.

Add your own feedback and questions here:
You are equally welcome to be positive or negative about any paper but please be polite. If you are being critical you must mention at least one specific error, otherwise your comment will be deleted as unhelpful.

comments powered by Disqus