The application of the herbomineral formulations in general skin health are increasing day-by-day due to the excellent outcomes without any adverse effects. This study was designed to evaluate the influence of The Trivedi Effect®-Consciousness Energy Healing Treatment on an herbomineral formulation and cell medium against various skin health parameters. The test formulation consists of minerals (zinc chloride, sodium selenate, and sodium molybdate) and L-ascorbic acid along with herbal extracts, Centella asiatica, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two equal parts, one was treated with a Biofield Treatment (BT) by Dezi Ann Koster and denoted as treated, while the other part was coded as the untreated (UT) groups. MTT assay results showed that test formulation was safe and nontoxic with more than 89% cell viability in the tested cell lines. BrdU assay showed an improved cell proliferation by 2.82% in the UT-DMEM + BT-Test formulation group compared with the untreated group. The level of collagen was significantly increased by 32.42%, 33.64%, and 29.13% at 2.5, 1.25 and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation, while34.17%, 26.73%, and 17.56% increased at 2.5, 1.25 and 0.625 µg/mL, respectively in BT-DMEM + BT-Test formulation group compared with the untreated group. Elastin level was increased by 408.6% at concentration of 0.625 µg/mL in UT-DMEM + BT-Test formulation group compared with untreated group. However, hyaluronic acid (HA) level was increased by 31.88%, 15.52%, and 58.29% at 2.5, 1.25, and 0.625 µg/mL, respectively in the BT-DMEM + BT-Test formulation group compared with untreated group. Besides, melanin synthesis was significantly inhibited by 16.09% and 18.93% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 0.125 µg/mL compared with the untreated group. Anti-wrinkling activity in HFF-1 cells showed an improved cell viability by 5.49% and 11.26% at 1.25 and 0.625 µg/mL, respectively in BT-DMEM + BT-Test formulation group compared with the untreated group. Wound healing scratch assay results showed a significant healing rate by 5% and 10% in HFF-1 and HaCaT cells lines, respectively with high cellular migration of fibroblast and keratinocytes. Overall, it can be concluded that the Biofield Energy Healing (The Trivedi Effect®) based test formulation and cell medium could be helpful against various skin disorders and can be used in psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating agent.
Comments: 11 Pages.
[v1] 2017-07-06 02:20:14
Unique-IP document downloads: 18 times
Vixra.org is a pre-print repository rather than a journal. Articles hosted may not yet have been verified by peer-review and should be treated as preliminary. In particular, anything that appears to include financial or legal advice or proposed medical treatments should be treated with due caution. Vixra.org will not be responsible for any consequences of actions that result from any form of use of any documents on this website.
Add your own feedback and questions here:
You are equally welcome to be positive or negative about any paper but please be polite. If you are being critical you must mention at least one specific error, otherwise your comment will be deleted as unhelpful.