Oxidative stress causes serious skin damage that is characterized by ageing, wrinkling, roughness, laxity and pigmentation. In the present work, the impact of Biofield Energy (The Trivedi Effect®-Consciousness Energy Healing) Treatment on the herbomineral test formulation and cell medium (DMEM) was evaluated for skin health parameters. The test formulation was consisted of minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two parts. One part received the Biofield Energy Treatment by Jagdish Singh and was termed as the Biofield Treated (BT) sample, while other was denoted as the untreated (UT) samples. MTT assay showed that test formulation was found safe and nontoxic with greater than 75% cell viability against various tested concentrations. Cell proliferation data using BrdU method showed an improved cell proliferation by 149.13% and 118.80% at 8.75 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the untreated group. The collagen level was significantly increased by 28.14% and 44.45% at 1.25 and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The elastin level was increased by 10.38%, 14.66%, and 48.24% at 2.5, 1.25, and 0.625 µg/mL, respectively in the BT-DMEM + UT-Test formulation group, compared with the untreated group. Moreover, melanin synthesis was significantly inhibited by 5.93%, 1.43%, and 1.43% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.0625 µg/mL compared with the untreated group. However, melanin synthesis was decreased by 11.71% and 15.75% at 0.125 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively. Anti-wrinkling effects exhibited improved cell viability by 17.19% and 28.68% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively in HFF-1 cells compared with the untreated group. Wound healing activity using scratch assay showed a significantly improved healing rate upto 5% in the HFF-1 and HaCaT cells lines in the Biofield Energy Healing based test formulation. Overall, the data suggests that The Trivedi Effect® treated test formulation and DMEM has the capacity to improve the skin health and suggests its use in psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, and many more skin diseases.
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[v1] 2017-07-06 02:21:33
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